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验证数据展示

  • WB analysis using CL750-66051

    WB analysis using CL750-66051

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-66051 (Albumin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using CL750-66051

    WB analysis using CL750-66051

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-66051 (Albumin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

CoraLite® Plus 750-conjugated Albumin Monoclonal antibody

Albumin Monoclonal Antibody for WB
Cat No. CL750-66051

产品说明书

CloneNo. 4A1C11

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, pig

应用

WB

别名

ALB, Serum albumin, HSA, 4A1C11

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 750
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, human plasma
Planning an IHC experiment? We recommend our IHCeasy ALB Ready-To-Use IHC Kit. ALB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66051-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-66051 targets Albumin in WB applications and shows reactivity with human, rat, pig samples.

经测试应用 WB Application Description
经测试反应性 human, rat, pig
免疫原

CatNo: Ag9885

Product name: Recombinant human Albumin protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 256-609 aa of BC034023

Sequence: SKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 albumin
别名 ALB, Serum albumin, HSA, 4A1C11
计算分子量 609 aa, 69 kDa
观测分子量 66 kDa
GenBank蛋白编号BC034023
基因名称 Albumin
Gene ID (NCBI) 213
RRIDAB_3084887
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP02768
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071) In additon, glycated serum albumin is also a potential diabetes biomarker.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 Albumin antibody CL750-66051Download protocol
Standard Protocols
Click here to view our Standard Protocols
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