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验证数据展示

  • WB analysis using 84819-5-RR

    WB analysis using 84819-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84819-5-RR (Aggrecan antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 84819-5-RR

    IHC staining of human lung cancer using 84819-5-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84819-5-RR (Aggrecan antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACAN antibody included
  • IHC staining of human lung cancer using 84819-5-RR

    IHC staining of human lung cancer using 84819-5-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84819-5-RR (Aggrecan antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACAN antibody included
  • Affinity of 84819-5-RR

    Affinity of 84819-5-RR

    Biolayer interferometry (BLl) kinetic assays of 88419-5-RR against Human Aggrecan were performed. The affinity constant is 0.124 nM.

Aggrecan Recombinant monoclonal antibody

Aggrecan Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84819-5-RR

产品说明书

CloneNo. 250777C8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ACAN, AGC1, AGCAN, Aggrecan 1, Aggrecan core protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse cartilage tissue, rat auricular cartilage tissue
Positive IHC detected inhuman lung cancer tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ACAN Ready-To-Use IHC Kit. ACAN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84819-5-RR targets Aggrecan in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg2994

Product name: Recombinant Human ACAN protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 20-675 aa of NM_013227.4

Sequence: VETSDHDNSLSVSIPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQAGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYVHANQTGYPDPSSRYDAICYTGEDFVDIPENFFGVGGEEDITVQTVTWPDMELPLPRNITEGEARGSVILTVKPIFEVSPSPLEPEEPFTFAPEIGATAFAEVENETGEATRPWGFPTPGLGPATAFTSEDLVVQVTAVPGQPHLPGGVVFHYRPGPTRYSLTFEEAQQACLRTGAVIASPEQLQAAYEAGYEQCDAGWLRDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPSTETYDVYCFVDRLEGEVFFATRLEQFTFQEALEFCESHNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLPDPLSRHHAFCFR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aggrecan
别名 ACAN, AGC1, AGCAN, Aggrecan 1, Aggrecan core protein 2
计算分子量261 kDa
观测分子量140-150 kDa
GenBank蛋白编号NM_013227.4
基因名称 ACAN
Gene ID (NCBI) 176
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP16112-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

实验方案

Product Specific Protocols
IHC protocol for Aggrecan antibody 84819-5-RRDownload protocol
WB protocol for Aggrecan antibody 84819-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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