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验证数据展示

  • IF Staining of mouse liver using CL488-85121-4

    IF Staining of mouse liver using CL488-85121-4

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CoraLite® Plus 488 Adiponectin receptor antibody (CL488-85121-4, Clone: 242777F3 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 488-conjugated Adiponectin receptor Recombinant antibody

Adiponectin receptor Recombinant Antibody for IF-P
Cat No. CL488-85121-4

产品说明书

CloneNo. 242777F3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P

别名

ADIPOR2, 242777F3, ACDCR2, adiponectin receptor 2, Adiponectin receptor protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse liver tissue
For other applications, we recommend the unconjugated version of this antibody, 85121-4-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-85121-4 targets Adiponectin receptor in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 Adiponectin receptor fusion protein Ag5744 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 adiponectin receptor 2
别名 ADIPOR2, 242777F3, ACDCR2, adiponectin receptor 2, Adiponectin receptor protein 2
计算分子量 44 kDa
GenBank蛋白编号BC051858
基因名称 Adiponectin receptor
Gene ID (NCBI) 79602
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ86V24
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adiponectin is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Two receptors for adiponectin (ADIPOR1 and ADIPOR2) have been identified. They mediate increased AMPK, MAPK, and PPARA ligand activity in response to adiponectin. AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. The human ADIPOR2 gene maps to chromosome 12p13.31, and encodes a 386-amino acid protein with a molecular weight of 44 kDa. AdipoR2 may also exist as stable ~ 60 kDa dimers (PMID: 18842004). This antibody raised against 1-147aa of human AdipoR2 may cross-react with AdipoR1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Adiponectin receptor antibody CL488-85121-4Download protocol
Standard Protocols
Click here to view our Standard Protocols