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验证数据展示

  • Dot Blot experiment using 82828-14-RR

    Dot Blot experiment using 82828-14-RR

    Dot blot analysis was used to confirm the specificity of 82828-14-RR Acetyl-Histone H3(Lys36) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  • ChIP experiment of HeLa using 82828-14-RR

    ChIP experiment of HeLa using 82828-14-RR

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of HIST1H3A (82828-14-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • WB analysis using 82828-14-RR

    WB analysis using 82828-14-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82828-14-RR (Acetyl-Histone H3 (Lys36) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Acetyl-Histone H3 (Lys36) Recombinant monoclonal antibody

Acetyl-Histone H3 (Lys36) Uni-rAb® Recombinant Antibody for WB, Dot Blot, ELISA, ChIP-qPCR
Cat No. 82828-14-RR

产品说明书

CloneNo. 250370A9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Dot Blot, ELISA, ChIP-qPCR

别名

Ac Histone H3, Ac Histone H3 (Lys36), Ac Histone H3 Lys36, Ac Histone H3-Lys36, Acetyl Histone

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTrichostatin A treated NIH/3T3 cells
Positive Dot Blot detected inN/A N/A
Positive ChIP-qPCR detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
DOT BLOTDOT BLOT : 1:10-1:100
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82828-14-RR targets Acetyl-Histone H3 (Lys36) in WB, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

经测试应用 WB, Dot Blot, ELISA, ChIP-qPCR Application Description
经测试反应性 human, mouse
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H3a
别名 Ac Histone H3, Ac Histone H3 (Lys36), Ac Histone H3 Lys36, Ac Histone H3-Lys36, Acetyl Histone
观测分子量15 kDa
GenBank蛋白编号BC066245
基因名称 HIST1H3A
Gene ID (NCBI) 8350
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP68431
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

H3K36ac peaks at the 5' end of genes,manily on the two nucleosomeus immediately distal to the transcription start site, independently of gene length. H3K36ac and H3K36mac showed negative creastalk, which is mediated by GCN3 and the histone methyl transferase SDX8. Although H3K36ac is associated with gene activity, there is no linear relationship between H3K36ac and transcript levels, suggesting that H3K36ac is a binary indicator of transcription.

实验方案

Product Specific Protocols
WB protocol for Acetyl-Histone H3 (Lys36) antibody 82828-14-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols