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验证数据展示

  • Dot Blot experiment of peptide using 82838-2-RR

    Dot Blot experiment of peptide using 82838-2-RR

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys23) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  • ChIP experiment of HeLa using 82838-2-RR

    ChIP experiment of HeLa using 82838-2-RR

    Chromatin was prepared from HeLa cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys23) (82838-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • IHC staining of mouse testis using 82838-2-RR

    IHC staining of mouse testis using 82838-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82838-2-RR (HIST1H3A antibody) at dilution of 1:400 (under 10x lens).

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IHC staining of mouse testis using 82838-2-RR

    IHC staining of mouse testis using 82838-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82838-2-RR (Acetyl-Histone H3 (Lys23) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IHC staining of rat testis using 82838-2-RR

    IHC staining of rat testis using 82838-2-RR

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 82838-2-RR (Acetyl-Histone H3 (Lys23) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IF Staining of HeLa using 82838-2-RR

    IF Staining of HeLa using 82838-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H3 (Lys23) antibody (82838-2-RR, Clone: 241137E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Acetyl-Histone H3 (Lys23) Recombinant antibody

Acetyl-Histone H3 (Lys23) Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ChIP, Dot Blot, ELISA
Cat No. 82838-2-RR

产品说明书

CloneNo. 241137E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ChIP, Dot Blot, ELISA

别名

Acetyl Histone, H3 Ac Lys23, H3 Acetyl K23, H3 Acetyl Lys23, H3 Acetyl Lysine23

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive Dot Blot detected inpeptide
Positive ChIP detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
DOT BLOTDOT BLOT : 1:10-1:100
Chromatin immunoprecipitation (ChIP)CHIP : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82838-2-RR targets Acetyl-Histone H3 (Lys23) in IHC, IF/ICC, ChIP, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ChIP, Dot Blot, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H3a
别名 Acetyl Histone, H3 Ac Lys23, H3 Acetyl K23, H3 Acetyl Lys23, H3 Acetyl Lysine23
GenBank蛋白编号BC066245
基因名称 HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_3670572
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP68431
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for Acetyl-Histone H3 (Lys23) antibody 82838-2-RRDownload protocol
IF protocol for Acetyl-Histone H3 (Lys23) antibody 82838-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols