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验证数据展示

  • Dot Blot experiment of peptide using 84549-1-RR

    Dot Blot experiment of peptide using 84549-1-RR

    Dot blot analysis was used to confirm the specificity of 84549-1-RR Acetyl-Histone H2B (Lys20) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

  • ChIP experiment of HeLa using 84549-1-RR

    ChIP experiment of HeLa using 84549-1-RR

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 3 µg of Acetyl-Histone H2B (Lys20) (84549-1-RR) or 3 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • WB analysis of NIH/3T3 using 84549-1-RR

    WB analysis of NIH/3T3 using 84549-1-RR

    Trichostatin A treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84549-1-RR (Acetyl-Histone H2B (Lys20) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • IF Staining of HeLa using 84549-1-RR

    IF Staining of HeLa using 84549-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2B (Lys20) antibody (84549-1-RR, Clone: 241204H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of NIH/3T3 using 84549-1-RR

    IF Staining of NIH/3T3 using 84549-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated NIH/3T3 cells using Acetyl-Histone H2B (Lys20) antibody (84549-1-RR, Clone: 241204H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 84549-1-RR

    IF Staining of HeLa using 84549-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H2B (Lys20) antibody (84549-1-RR, Clone: 241204H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Acetyl-Histone H2B (Lys20) Recombinant monoclonal antibody

Acetyl-Histone H2B (Lys20) Uni-rAb® Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR
Cat No. 84549-1-RR

产品说明书

CloneNo. 241204H8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

别名

Ac Histone H2B, Ac Histone H2B (Lys20), Ac Histone H2B Lys20, Ac Histone H2B-Lys20, Acetyl Histone

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTrichostatin A treated NIH/3T3 cells
Positive IF/ICC detected inHeLa cells, Trichostatin A treated HeLa cells, Trichostatin A treated NIH/3T3 cells
Positive Dot Blot detected inpeptide
Positive ChIP-qPCR detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
DOT BLOTDOT BLOT : 1:10-1:100
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84549-1-RR targets Acetyl-Histone H2B (Lys20) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 2, H2be
别名 Ac Histone H2B, Ac Histone H2B (Lys20), Ac Histone H2B Lys20, Ac Histone H2B-Lys20, Acetyl Histone
计算分子量 14 kDa
观测分子量15 kDa
GenBank蛋白编号BC005827
基因名称 Histone H2B
Gene ID (NCBI) 8349
RRIDAB_3672056
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ16778
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for Acetyl-Histone H2B (Lys20) antibody 84549-1-RRDownload protocol
WB protocol for Acetyl-Histone H2B (Lys20) antibody 84549-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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