• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • Dot Blot experiment of peptide using 84446-2-RR

    Dot Blot experiment of peptide using 84446-2-RR

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2B (Lys16) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  • WB analysis of HSC-T6 using 84446-2-RR

    WB analysis of HSC-T6 using 84446-2-RR

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 84446-2-RR (Acetyl-Histone H2B (Lys16) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 84446-2-RR

    WB analysis of NIH/3T3 using 84446-2-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84446-2-RR (Acetyl-Histone H2B (Lys16) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84446-2-RR

    IF Staining of HeLa using 84446-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2B (Lys16) antibody (84446-2-RR, Clone: 241194C7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IF Staining of C6 using 84446-2-RR

    IF Staining of C6 using 84446-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated C6 cells using Acetyl-Histone H2B (Lys16) antibody (84446-2-RR, Clone: 241194C7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of NIH/3T3 using 84446-2-RR

    IF Staining of NIH/3T3 using 84446-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated NIH/3T3 cells using Acetyl-Histone H2B (Lys16) antibody (84446-2-RR, Clone: 241194C7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Acetyl-Histone H2B (Lys16) Recombinant antibody

Acetyl-Histone H2B (Lys16) Uni-rAbTM Recombinant Antibody for IF/ICC, Dot Blot, ELISA
Cat No. 84446-2-RR

产品说明书

CloneNo. 241194C7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, Dot Blot, ELISA

别名

Acetyl Histone, H2B Ac Lys16, H2B Acetyl K16, H2B Acetyl Lys16, H2B Acetyl Lysine16

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHeLa cells, Trichostatin A treated C6 cells, Trichostatin A treated NIH/3T3 cells
Positive Dot Blot detected inpeptide

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84446-2-RR targets Acetyl-Histone H2B (Lys16) in IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, Dot Blot, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 2, H2be
别名 Acetyl Histone, H2B Ac Lys16, H2B Acetyl K16, H2B Acetyl Lys16, H2B Acetyl Lysine16
计算分子量 14 kDa
GenBank蛋白编号BC005827
基因名称 Histone H2B
Gene ID (NCBI) 8349
RRIDAB_3671972
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ16778
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Acetyl-Histone H2B (Lys16) antibody 84446-2-RRDownload protocol
IF protocol for Acetyl-Histone H2B (Lys16) antibody 84446-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols