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验证数据展示

  • Dot Blot experiment of peptide using 84446-1-RR (same clone as 84446-1-PBS)

    Dot Blot experiment of peptide using 84446-1-RR (same clone as 84446-1-PBS)

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2B (Lys16) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 84446-1-PBS in a different storage buffer formulation.

  • ChIP experiment of HeLa using 84446-1-RR (same clone as 84446-1-PBS)

    ChIP experiment of HeLa using 84446-1-RR (same clone as 84446-1-PBS)

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H2B (Lys16) (84446-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 84446-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 84446-1-RR (same clone as 84446-1-PBS)

    WB analysis of NIH/3T3 using 84446-1-RR (same clone as 84446-1-PBS)

    Trichostatin A treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84446-1-RR (Acetyl-Histone H2B (Lys16) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84446-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 84446-1-RR (same clone as 84446-1-PBS)

    WB analysis of HSC-T6 using 84446-1-RR (same clone as 84446-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 84446-1-RR (Acetyl-Histone H2B (Lys16) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84446-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 84446-1-RR (same clone as 84446-1-PBS)

    WB analysis of NIH/3T3 using 84446-1-RR (same clone as 84446-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84446-1-RR (Acetyl-Histone H2B (Lys16) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84446-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84446-1-RR (same clone as 84446-1-PBS)

    IF Staining of HeLa using 84446-1-RR (same clone as 84446-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H2B antibody (84446-1-RR, Clone: 241194H2 ) at dilution of 1:850 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 84446-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84446-1-RR (same clone as 84446-1-PBS)

    IF Staining of HeLa using 84446-1-RR (same clone as 84446-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H2B (Lys16) antibody (84446-1-RR, Clone: 241194H2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84446-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 84446-1-RR (same clone as 84446-1-PBS)

    IF Staining of NIH/3T3 using 84446-1-RR (same clone as 84446-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated NIH/3T3 cells using Acetyl-Histone H2B (Lys16) antibody (84446-1-RR, Clone: 241194H2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84446-1-PBS in a different storage buffer formulation.

Acetyl-Histone H2B (Lys16) Recombinant monoclonal antibody, PBS Only

Acetyl-Histone H2B (Lys16) Uni-rAb® Recombinant Antibody for WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR
Cat No. 84446-1-PBS

产品说明书

CloneNo. 241194H2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

别名

Acetyl Histone, H2B Ac Lys16, H2B Acetyl K16, H2B Acetyl Lys16, H2B Acetyl Lysine16

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84446-1-PBS targets Acetyl-Histone H2B (Lys16) in WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 2, H2be
别名 Acetyl Histone, H2B Ac Lys16, H2B Acetyl K16, H2B Acetyl Lys16, H2B Acetyl Lysine16
计算分子量 14 kDa
观测分子量15 kDa
GenBank蛋白编号BC005827
基因名称 Histone H2B
Gene ID (NCBI) 8349
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ16778
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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