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验证数据展示

  • Dot Blot experiment of peptide using 84551-1-RR (same clone as 84551-1-PBS)

    Dot Blot experiment of peptide using 84551-1-RR (same clone as 84551-1-PBS)

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2B (Lys120) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 84551-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84551-1-RR (same clone as 84551-1-PBS)

    IF Staining of HeLa using 84551-1-RR (same clone as 84551-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H2B (Lys120) antibody (84551-1-RR, Clone: 241556E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84551-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 84551-1-RR (same clone as 84551-1-PBS)

    WB analysis of NIH/3T3 using 84551-1-RR (same clone as 84551-1-PBS)

    Trichostatin A treated NIH/3T3 cells and untreated cells were subjected to SDS PAGE followed by western blot with 84551-1-RR (Acetyl-Histone H2B (Lys120) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 84551-1-PBS in a different storage buffer formulation.

Acetyl-Histone H2B (Lys120) Recombinant antibody, PBS Only

Acetyl-Histone H2B (Lys120) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Dot Blot, Indirect ELISA
Cat No. 84551-1-PBS

产品说明书

CloneNo. 241556E7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Dot Blot, Indirect ELISA

别名

Acetyl Histone, H2B Ac Lys120, H2B Acetyl K120, H2B Acetyl Lys120, H2B Acetyl Lysine120

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84551-1-PBS targets Acetyl-Histone H2B (Lys120) in WB, IF/ICC, Dot Blot, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Dot Blot, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 2, H2be
别名 Acetyl Histone, H2B Ac Lys120, H2B Acetyl K120, H2B Acetyl Lys120, H2B Acetyl Lysine120
计算分子量 14 kDa
观测分子量25 kDa
GenBank蛋白编号BC005827
基因名称 Histone H2B
Gene ID (NCBI) 8349
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ16778
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).