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验证数据展示

  • Dot Blot experiment of / using 86731-1-RR

    Dot Blot experiment of / using 86731-1-RR

    Dot blot analysis was used to confirm the specificity of 86731-1-RR Acetyl-Histone H2B (Lys12) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  • ChIP experiment of HeLa using 86731-1-RR

    ChIP experiment of HeLa using 86731-1-RR

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H2B (Lys12) (86731-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • WB analysis of NIH/3T3 using 86731-1-RR

    WB analysis of NIH/3T3 using 86731-1-RR

    Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86731-1-RR (Acetyl-Histone H2B (Lys12) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control.

  • WB analysis of HeLa using 86731-1-RR

    WB analysis of HeLa using 86731-1-RR

    Trichostatin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 86731-1-RR (Acetyl-Histone H2B (Lys12) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control.

  • IHC staining of human placenta using 86731-1-RR

    IHC staining of human placenta using 86731-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86731-1-RR (Acetyl-Histone H2B (Lys12) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 86731-1-RR

    IHC staining of human placenta using 86731-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86731-1-RR (Acetyl-Histone H2B (Lys12) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of NIH/3T3 using 86731-1-RR

    FC experiment of NIH/3T3 using 86731-1-RR

    1x10^6 Trichostatin A treated NIH/3T3 cells were intracellularly stained with 0.13 ug Acetyl-Histone H2B (Lys12) Recombinant monoclonal antibody (86731-1-RR, Clone:250005E8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Acetyl-Histone H2B (Lys12) Recombinant monoclonal antibody

Acetyl-Histone H2B (Lys12) Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), Dot blot, ELISA, ChIP-qPCR
Cat No. 86731-1-RR

产品说明书

CloneNo. 250005E8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), Dot blot, ELISA, ChIP-qPCR

别名

Ac Histone H2B, Ac Histone H2B (Lys12), Ac Histone H2B Lys12, Ac Histone H2B-Lys12, Acetyl Histone

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTrichostatin A treated NIH/3T3 cells, Trichostatin A treated HeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inTrichostatin A treated NIH/3T3 cells
Positive Dot blot detected in/ /
Positive ChIP-qPCR detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
DOT BLOTDOT BLOT : 1:10-1:100
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86731-1-RR targets Acetyl-Histone H2B (Lys12) in WB, IHC, FC (Intra), Dot blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), Dot blot, ELISA, ChIP-qPCR Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 2, H2be
别名 Ac Histone H2B, Ac Histone H2B (Lys12), Ac Histone H2B Lys12, Ac Histone H2B-Lys12, Acetyl Histone
计算分子量 14 kDa
观测分子量15 kDa
GenBank蛋白编号BC005827
基因名称 Histone H2B
Gene ID (NCBI) 8349
RRIDAB_3745087
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16778
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.

实验方案

Product Specific Protocols
FC protocol for Acetyl-Histone H2B (Lys12) antibody 86731-1-RRDownload protocol
IHC protocol for Acetyl-Histone H2B (Lys12) antibody 86731-1-RRDownload protocol
WB protocol for Acetyl-Histone H2B (Lys12) antibody 86731-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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