AZI2/NAP1 Polyclonal antibody
AZI2/NAP1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IP detected in | HEK-293 cells, HepG2 cells |
| Positive IHC detected in | mouse testis tissue, human pancreas tissue, human stomach tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15042-1-AP targets AZI2/NAP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | AZI2/NAP1 fusion protein Ag6892 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | 5-azacytidine induced 2 |
| Synonyms | 5 azacytidine induced 2, AZ2, AZI2, AZI2/NAP1, Nak associated protein 1, NAP1, TILP |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC001139 |
| Gene Symbol | AZI2 |
| Gene ID (NCBI) | 64343 |
| RRID | AB_2878103 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H6S1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AZI2, also known as NAP1, is a TNF receptor (TNFR)-associated factor family member-associated NF-κB activator-binding kinase 1-binding protein that regulates the production of IFNs. It is an adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AZI2/NAP1 antibody 15042-1-AP | Download protocol |
| IHC protocol for AZI2/NAP1 antibody 15042-1-AP | Download protocol |
| IF protocol for AZI2/NAP1 antibody 15042-1-AP | Download protocol |
| IP protocol for AZI2/NAP1 antibody 15042-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res FIP200 suppresses immune checkpoint therapy responses in breast cancers by limiting AZI2/TBK1/IRF signaling independent of its canonical autophagy function. | ||
PLoS Pathog MAVS activates TBK1 and IKKε through TRAFs in NEMO dependent and independent manner. | ||
Front Immunol Increased IKKϵ protein stability ensures efficient type I interferon responses in conditions of TBK1 deficiency | ||
Autophagy AZI2 mediates TBK1 activation at unresolved selective autophagy cargo receptor complexes with implications for CD8 T-cell infiltration in breast cancer | ||
J Cell Biol NAK-associated protein 1/NAP1 activates TBK1 to ensure accurate mitosis and cytokinesis |
