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验证数据展示

  • WB analysis using 86809-3-RR (same clone as 86809-3-PBS)

    WB analysis using 86809-3-RR (same clone as 86809-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86809-3-RR (AZI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86809-3-PBS in a different storage buffer formulation.

  • WB analysis using 86809-3-RR (same clone as 86809-3-PBS)

    WB analysis using 86809-3-RR (same clone as 86809-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86809-3-RR (AZI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86809-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86809-3-RR (same clone as 86809-3-PBS)

    IF Staining of HeLa using 86809-3-RR (same clone as 86809-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AZI1 antibody (86809-3-RR, Clone: 251839D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86809-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86809-3-RR

    Affinity and Kinetic Characterization of 86809-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86809-3-RR against Human AZI1 were performed. The affinity constant is 0.704 nM.

AZI1 Recombinant monoclonal antibody, PBS Only

AZI1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86809-3-PBS

产品说明书

CloneNo. 251839D3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

5 azacytidine induced 1, 5-azacytidine-induced protein 1, AZ1, CEP131, KIAA1118

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86809-3-PBS targets AZI1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag22699

Product name: Recombinant human AZI1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 730-1080 aa of BC136578

Sequence: HEAELLQSDERASQRCLRQAEELREQLEREKEALGQQERERARQRFQQHLEQEQWALQQQRQRLYSEVAEERERLGQQAARQRAELEELRQQLEESSSALTRALRAEFEKGREEQERRHQMELNTLKQQLELERQAWEAGRTRKEEAWLLNREQELREEIRKGRDKEIELVIHRLEADMALAKEESEKAAESRIKRLRDKYEAELSELEQSERKLQERCSELKGQLGEAEGENLRLQGLVRQKERALEDAQAVNEQLSSERSNLAQVIRQEFEDRLAASEEETRQAKAELATLQARQQLELEEVHRRVKTALARKEEAVSSLRTQHEAAVKRADHLEELLEQHRRPTPSTK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 5-azacytidine induced 1
别名 5 azacytidine induced 1, 5-azacytidine-induced protein 1, AZ1, CEP131, KIAA1118
计算分子量 1083 aa, 122 kDa
观测分子量117-130 kDa
GenBank蛋白编号BC136578
基因名称 AZI1
Gene ID (NCBI) 22994
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UPN4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AZI1, also named as CEP131 and KIAA1118, may play a role in spermatogenesis. It is located at the ciliary base and required for cilium formation.

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