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验证数据展示

  • WB analysis of human plasma using 66178-1-Ig

    WB analysis of human plasma using 66178-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66178-1-Ig (AZGP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 66178-1-Ig

    IHC staining of human breast cancer using 66178-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with AZGP1 antibody included
  • IHC staining of human breast cancer using 66178-1-Ig

    IHC staining of human breast cancer using 66178-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with AZGP1 antibody included
  • IF Staining of human breast cancer using 66178-1-Ig

    IF Staining of human breast cancer using 66178-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Zinc Alpha 2 Glycoprotein antibody (66178-1-Ig, Clone: 4B8A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Zinc Alpha 2 Glycoprotein Monoclonal antibody

Zinc Alpha 2 Glycoprotein Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66178-1-Ig

产品说明书

CloneNo. 4B8A4
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

AZGP1, Zinc-alpha-2-glycoprotein, 4B8A4, ZA2G, ZAG

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue
Planning an IHC experiment? We recommend our IHCeasy AZGP1 Ready-To-Use IHC Kit. AZGP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66178-1-Ig targets Zinc Alpha 2 Glycoprotein in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag5661

Product name: Recombinant human AZGP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-298 aa of BC033830

Sequence: MVRMVPVLLSLLLLLGPAVPQENQDGRYSLTYIYTGLSKHVEDVPAFQALGSLNDLQFFRYNSKDRKSQPMGLWRQVEGMEDWKQDSQLQKAREDIFMETLKDIVEYYNDSNGSHVLQGRFGCEIENNRSSGAFWKYYYDGKDYIEFNKEIPAWVPFDPAAQITKQKWEAEPVYVQRAKAYLEEECPATLRKYLKYSKNILDRQDPPSVVVTSHQAPGEKKKLKCLAYDFYPGKIDVHWTRAGEVQEPELRGDVLHNGNGTYQSWVVVAVPPQDTAPYSCHVQHSSLAQPLVVPWEAS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 alpha-2-glycoprotein 1, zinc-binding
别名 AZGP1, Zinc-alpha-2-glycoprotein, 4B8A4, ZA2G, ZAG
计算分子量 298 aa, 34 kDa
观测分子量 41 kDa
GenBank蛋白编号BC033830
基因名称 Zinc Alpha 2 Glycoprotein
Gene ID (NCBI) 563
RRIDAB_2881573
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP25311
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc-alpha-2-glycoprotein (AZGP1), a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract, and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).

实验方案

Product Specific Protocols
IF protocol for Zinc Alpha 2 Glycoprotein antibody 66178-1-IgDownload protocol
IHC protocol for Zinc Alpha 2 Glycoprotein antibody 66178-1-IgDownload protocol
WB protocol for Zinc Alpha 2 Glycoprotein antibody 66178-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Onco Targets Ther

Reduction of AZGP1 predicts poor prognosis in esophageal squamous cell carcinoma patients in Northern China.

Authors - Hong Tang
View Article
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