验证数据展示
经过测试的应用
| Positive WB detected in | human plasma, MCF-7 cells |
| Positive IHC detected in | mouse liver tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 4 publications below |
产品信息
13399-1-AP targets Zinc Alpha 2 Glycoprotein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | Zinc Alpha 2 Glycoprotein fusion protein Ag4232 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | alpha-2-glycoprotein 1, zinc-binding |
| 别名 | AZGP1, Zinc-alpha-2-glycoprotein, Zn alpha 2 GP, Zn alpha 2 glycoprotein, ZAG |
| 计算分子量 | 298 aa, 34 kDa |
| 观测分子量 | 41 kDa |
| GenBank蛋白编号 | BC033830 |
| 基因名称 | Zinc Alpha 2 Glycoprotein |
| Gene ID (NCBI) | 563 |
| RRID | AB_2062582 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P25311 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Zinc-alpha-2-glycoprotein (AZGP1) is a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Zinc Alpha 2 Glycoprotein antibody 13399-1-AP | Download protocol |
| IHC protocol for Zinc Alpha 2 Glycoprotein antibody 13399-1-AP | Download protocol |
| IF protocol for Zinc Alpha 2 Glycoprotein antibody 13399-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Mucus concentration-dependent biophysical abnormalities unify submucosal gland and superficial airway dysfunction in cystic fibrosis. | ||
Int J Cancer Reduced AZGP1 expression is an independent predictor of early PSA recurrence and associated with ERG fusion positive and PTEN deleted prostate cancers. | ||
J Transl Med Decreased expression of zinc-alpha2-glycoprotein in hepatocellular carcinoma associates with poor prognosis. | ||
Proteome Sci Identification of low-abundance proteins via fractionation of the urine proteome with weak anion exchange chromatography. | ||
