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验证数据展示

  • WB analysis using 24704-1-AP (same clone as 24704-1-PBS)

    WB analysis using 24704-1-AP (same clone as 24704-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24704-1-AP (AWAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24704-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 24704-1-AP (same clone as 24704-1-PBS)

    IHC staining of mouse liver using 24704-1-AP (same clone as 24704-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24704-1-AP (AWAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24704-1-PBS in a different storage buffer formulation.

AWAT1 Polyclonal antibody, PBS Only

AWAT1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24704-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

DGA2, DGAT2L3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24704-1-PBS targets AWAT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20183

Product name: Recombinant human AWAT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 134-195 aa of BC153034

Sequence: LATLSWFFKIPFVREYLMAKGVCSVSQPAINYLLSHGTGNLVGIVVGGVGEALQSVPNTTTL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acyl-CoA wax alcohol acyltransferase 1
别名 DGA2, DGAT2L3
计算分子量 328 aa, 38 kDa
观测分子量45 kDa
GenBank蛋白编号BC153034
基因名称 AWAT1
Gene ID (NCBI) 158833
RRIDAB_3742123
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ58HT5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AWAT1 is an ER-localized acyltransferase specialized for wax ester biosynthesis in sebaceous and meibomian glands. By esterifying long-chain fatty acyl-CoAs with fatty alcohols, AWAT1 generates crucial lipids that maintain skin barrier function, sebaceous secretion, and tear film stability. Dysregulation of AWAT1 contributes to meibomian gland dysfunction, dry eye disease, and sebaceous lipid imbalance, positioning AWAT1 as a key enzymatic regulator of surface lipid homeostasis.

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