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验证数据展示

  • WB analysis using 86613-3-RR (same clone as 86613-3-PBS)

    WB analysis using 86613-3-RR (same clone as 86613-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86613-3-RR (ATXN2L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86613-3-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 86613-3-RR (same clone as 86613-3-PBS)

    IF Staining of Jurkat using 86613-3-RR (same clone as 86613-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ATXN2L antibody (86613-3-RR, Clone: 251578A9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86613-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86613-3-RR

    Affinity and Kinetic Characterization of 86613-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86613-3-RR against Human ATXN2L were performed. The affinity constant is 1.78 nM.

ATXN2L Recombinant monoclonal antibody, PBS Only

ATXN2L Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86613-3-PBS

产品说明书

CloneNo. 251578A9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

A2D, A2LG, A2LP, A2RP, ataxin 2 like

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86613-3-PBS targets ATXN2L in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag20581

Product name: Recombinant human ATXN2L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 712-1062 aa of BC082760

Sequence: SYIPQIHMGPAVQAPQMYPYPVSNSVPGQQGKYRGAKGSLPPQRSDQHQPASAPPMMQAAAAAGPPLVAATPYSSYIPYNPQQFPGQPAMMQPMAHYPSQPVFAPMLQSNPRMLTSGSHPQAIVSSSTPQYPSAEQPTPQALYATVHQSYPHHATQLHAHQPQPATTPTGSQPQSQHAAPSPVQHQAGQAPHLGSGQPQQNLYHPGALTGTPPSLPPGPSAQSPQSSFPQPAAVYAIHHQQLPHGFTNMAHVTQAHVQTGITAAPPPHPGAPHPPQVMLLHPPQSHGGPPQGAVPQSGVPALSASTPSPYPYIGHPQGEQPGQAPGFPGGADDRILQSHPSQQLPFHPPGN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ataxin 2-like
别名 A2D, A2LG, A2LP, A2RP, ataxin 2 like
计算分子量 1062 aa, 112 kDa
观测分子量150 kDa
GenBank蛋白编号BC082760
基因名称 ATXN2L
Gene ID (NCBI) 11273
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8WWM7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATXN2L is a paralog of Ataxin-2 (ATXN2) but without abnormal polyQ expended track, which is conserved in most of the ATXNs and drives the pathogenesis of neurodegeneration. It was reported that ATXN2L was widely expressed in immortalized cell lines, and ATXN2L-JAK2 fusion was found in CD4-positive T-cell lymphoma.

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