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Host
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Species Reactivity
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验证数据展示

  • WB analysis using 85288-2-RR (same clone as 85288-2-PBS)

    WB analysis using 85288-2-RR (same clone as 85288-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85288-2-RR (ATP7B-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85288-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 85288-2-RR (same clone as 85288-2-PBS)

    IHC staining of mouse liver using 85288-2-RR (same clone as 85288-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85288-2-RR (ATP7B-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85288-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 85288-2-RR (same clone as 85288-2-PBS)

    IHC staining of mouse liver using 85288-2-RR (same clone as 85288-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85288-2-RR (ATP7B-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85288-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 85288-2-RR (same clone as 85288-2-PBS)

    IF Staining of mouse liver using 85288-2-RR (same clone as 85288-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using ATP7B-Specific antibody (85288-2-RR, Clone: 242827B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85288-2-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 85288-2-RR (same clone as 85288-2-PBS)

    FC experiment of HepG2 using 85288-2-RR (same clone as 85288-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ATP7B-Specific Recombinant antibody (85288-2-RR, Clone:242827B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85288-2-PBS in a different storage buffer formulation.

    {{ptg:ProductFeaturesInfo}}

ATP7B-Specific Recombinant monoclonal antibody, PBS Only

ATP7B-Specific Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 85288-2-PBS

产品说明书

CloneNo. 242827B3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, FC (Intra), Indirect ELISA

别名

ATP7B, 242827B3, ATPase, Cu++ transporting, beta polypeptide, Copper-transporting ATPase 2, EC:7.2.2.8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85288-2-PBS targets ATP7B-Specific in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase, Cu++ transporting, beta polypeptide
别名 ATP7B, 242827B3, ATPase, Cu++ transporting, beta polypeptide, Copper-transporting ATPase 2, EC:7.2.2.8
计算分子量 157 kDa
观测分子量 150 kDa
GenBank蛋白编号NM_000053
基因名称 ATP7B
Gene ID (NCBI) 540
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35670
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP7B, also named as PWD, WC1 and WND, belongs to the cation transport ATPase (P-type) family and Type IB subfamily. ATP7B is involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. ATP7B catalyzes the reaction: ATP + H2O + Cu2+(In) = ADP + phosphate + Cu2+(Out). Defects in ATP7B are the cause of Wilson disease (WD). This antibody is specific to ATP7B.