验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, human kidney tissue |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human kidney tissue, human brain tissue, human heart tissue, human liver tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy ATP6V1G1 Ready-To-Use IHC Kit. ATP6V1G1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16143-1-AP targets ATP6V1G1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag9217 Product name: Recombinant human ATP6V1G1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-118 aa of BC008452 Sequence: MASQSQGIQQLLQAEKRAAEKVSEARKRKNRRLKQAKEEAQAEIEQYRLQREKEFKAKEAAALGSRGSCSTEVEKETQEKMTILQTYFRQNRDEVLDNLLAFVCDIRPEIHENYRING 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1 |
| 别名 | V-ATPase 13 kDa subunit 1, V ATPase subunit G 1, ATP6J, ATP6GL, ATP6G1 |
| 计算分子量 | 118 aa, 14 kDa |
| 观测分子量 | 14 kDa |
| GenBank蛋白编号 | BC008452 |
| 基因名称 | ATP6V1G1 |
| Gene ID (NCBI) | 9550 |
| RRID | AB_2062686 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75348 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ATP6V1G1 antibody 16143-1-AP | Download protocol |
| IHC protocol for ATP6V1G1 antibody 16143-1-AP | Download protocol |
| IP protocol for ATP6V1G1 antibody 16143-1-AP | Download protocol |
| WB protocol for ATP6V1G1 antibody 16143-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Physiol A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells. | ||
PLoS One Acidification of endothelial Weibel-Palade bodies is mediated by the vacuolar-type H+-ATPase. | ||
EBioMedicine A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes.
| ||
EBioMedicine Specific V-ATPase expression sub-classifies IDHwt lower-grade gliomas and impacts glioma growth in vivo. | ||
Autophagy Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate |
