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Species Reactivity
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验证数据展示

  • WB analysis using 87157-1-RR (same clone as 87157-1-PBS)

    WB analysis using 87157-1-RR (same clone as 87157-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87157-1-RR (ATP6V1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87157-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 87157-1-RR (same clone as 87157-1-PBS)

    IHC staining of human kidney using 87157-1-RR (same clone as 87157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 87157-1-RR (ATP6V1F antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87157-1-PBS in a different storage buffer formulation.

  • Affinity of 87157-1-RR

    Affinity of 87157-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87157-1-RR against Human ATP6V1F were performed. The affinity constant is 0.247 nM.

ATP6V1F Recombinant monoclonal antibody, PBS Only

ATP6V1F Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 87157-1-PBS

产品说明书

CloneNo. 252263C1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

ATP6S14, V ATPase 14 kDa subunit, V ATPase subunit F, V type proton ATPase subunit F, Vacuolar proton pump subunit F

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87157-1-PBS targets ATP6V1F in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12121

Product name: Recombinant human ATP6V1F protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-119 aa of BC107854

Sequence: MAGRGKLIAVIGDEDTVTGFLLGGIGELNKNRHPNFLVVEKDTTINEIEDTFRQFLNRDDIGIILINQYIAEMVRHALDAHQQSIPAVLEIPSKEHPYDAAKDSILRRARGMFTAEDLR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F
别名 ATP6S14, V ATPase 14 kDa subunit, V ATPase subunit F, V type proton ATPase subunit F, Vacuolar proton pump subunit F
计算分子量 119 aa, 13 kDa
观测分子量 14 kDa
GenBank蛋白编号BC107854
基因名称 ATP6V1F
Gene ID (NCBI) 9296
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16864
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP6V1F(V-type proton ATPase subunit F) is also named as ATP6S14, VATF and belongs to the V-ATPase F subunit family. It generates an electrochemical proton gradient that is acid and positive inside synaptic vesicles. ATP6V1F plays a major role as energizers of animal plasma membranes, especially apical plasma membranes of epithelial cells. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 14 kDa and 16 kDa.

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