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验证数据展示

  • WB analysis using 87157-1-RR

    WB analysis using 87157-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87157-1-RR (ATP6V1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 87157-1-RR

    IHC staining of human kidney using 87157-1-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 87157-1-RR (ATP6V1F antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity of 87157-1-RR

    Affinity of 87157-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87157-1-RR against Human ATP6V1F were performed. The affinity constant is 0.247 nM.

ATP6V1F Recombinant monoclonal antibody

ATP6V1F Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 87157-1-RR

产品说明书

CloneNo. 252263C1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ATP6S14, V ATPase 14 kDa subunit, V ATPase subunit F, V type proton ATPase subunit F, Vacuolar proton pump subunit F

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, A549 cells, Jurkat cells, MCF-7 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87157-1-RR targets ATP6V1F in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12121

Product name: Recombinant human ATP6V1F protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-119 aa of BC107854

Sequence: MAGRGKLIAVIGDEDTVTGFLLGGIGELNKNRHPNFLVVEKDTTINEIEDTFRQFLNRDDIGIILINQYIAEMVRHALDAHQQSIPAVLEIPSKEHPYDAAKDSILRRARGMFTAEDLR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F
别名 ATP6S14, V ATPase 14 kDa subunit, V ATPase subunit F, V type proton ATPase subunit F, Vacuolar proton pump subunit F
计算分子量 119 aa, 13 kDa
观测分子量 14 kDa
GenBank蛋白编号BC107854
基因名称 ATP6V1F
Gene ID (NCBI) 9296
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16864
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP6V1F(V-type proton ATPase subunit F) is also named as ATP6S14, VATF and belongs to the V-ATPase F subunit family. It generates an electrochemical proton gradient that is acid and positive inside synaptic vesicles. ATP6V1F plays a major role as energizers of animal plasma membranes, especially apical plasma membranes of epithelial cells. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 14 kDa and 16 kDa.

实验方案

Product Specific Protocols
IHC protocol for ATP6V1F antibody 87157-1-RRDownload protocol
WB protocol for ATP6V1F antibody 87157-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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