验证数据展示
经过测试的应用
| Positive WB detected in | JAR cells, pig brain tissue, pig cerebellum tissue, rabbit brain tissue, rabbit cerebellum tissue, rat kidney tissue, rat brain tissue, mouse kidney tissue, mouse brain tissue |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
68219-1-Ig targets ATP6V1B1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 文献引用反应性 | c.elegans |
| 免疫原 |
CatNo: Ag7405 Product name: Recombinant human ATP6V1B1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-327 aa of BC063411 Sequence: MAMEIDSRPGGLPGSSCNLGAAREHMQAVTRNYITHPRVTYRTVCSVNGPLVVLDRVKFAQYAEIVHFTLPDGTQRSGQVLEVAGTKAIVQVFEGTSGIDARKTTCEFTGDILRTPVSEDMLGRVFNGSGKPIDKGPVVMAEDFLDINGQPINPHSRIYPEEMIQTGISPIDVMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKAVLDYHDDNFAIVFAAMGVNMETARFFKSDFEQNGTMGNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLAT 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1 |
| 别名 | 1G10A8, ATP6B1, VATB, V-ATPase subunit B 1, VPP3 |
| 计算分子量 | 57 kDa |
| 观测分子量 | 56 kDa |
| GenBank蛋白编号 | BC063411 |
| 基因名称 | ATP6V1B1 |
| Gene ID (NCBI) | 525 |
| RRID | AB_2935307 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P15313 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ATP6V1B1 antibody 68219-1-Ig | Download protocol |
| WB protocol for ATP6V1B1 antibody 68219-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
