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验证数据展示

  • IHC staining of mouse kidney using 86321-1-RR

    IHC staining of mouse kidney using 86321-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:80000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of rat kidney using 86321-1-RR

    WB analysis of rat kidney using 86321-1-RR

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86321-1-RR

    WB analysis using 86321-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 86321-1-RR

    IHC staining of human kidney using 86321-1-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 86321-1-RR

    IHC staining of human kidney using 86321-1-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 86321-1-RR

    Affinity and Kinetic Characterization of 86321-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86321-1-RR against Human ATP6V0A4 were performed. The affinity constant is 0.715 nM.

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ATP6V0A4 Recombinant monoclonal antibody

ATP6V0A4 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 86321-1-RR

产品说明书

CloneNo. 250861E8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

A4, ATP6N1B, ATP6N2, RDRTA2, RTA1C

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman kidney tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86321-1-RR targets ATP6V0A4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16095

Product name: Recombinant human ATP6V0A4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 190-375 aa of BC109305

Sequence: ERMASFERLLWRICRGNVYLKFSEMDAPLEDPVTKEEIQKNIFIIFYQGEQLRQKIKKICDGFRATVYPCPERAVERREMLESVNVRLEDLITVITQTESHRQRLLQEAAANWHSWLIKVQKMKAVYHILNMCNIDVTQQCVIAEIWFPVADATRIKRALEQGMELSGSSMAPIMTTVQSKTAPPTFNRTNKFTAG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal V0 subunit a4
别名 A4, ATP6N1B, ATP6N2, RDRTA2, RTA1C
计算分子量 840 aa, 96 kDa
观测分子量 85 kDa
GenBank蛋白编号BC109305
基因名称 ATP6V0A4
Gene ID (NCBI) 50617
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9HBG4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ATP6V0A4 is a component of vacuolar-H+ATPase (V-ATPase) which is a multi-subunit enzyme that couples ATP hydrolysis to proton pumping across membranes. The V-ATPases are comprised of two major parts, the cytosolic V1 domain involved in ATP-binding and subsequent hydrolysis, and the membrane-associated V0 domain responsible for proton translocation. The V0 domain is composed of five subunits: a, c, c', c'' and d. The 'a' subunit of V0 domain has four isoforms : a1-a4. It has been found that mutations in ATP6V0A4(a4) are associated with distal renal tubular acidosis(dRTA) combined in some cases with progressive hearing loss leading to sensorineural deafness.

实验方案

Product Specific Protocols
WB protocol for ATP6V0A4 antibody 86321-1-RRDownload protocol
IHC protocol for ATP6V0A4 antibody 86321-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols