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验证数据展示

  • IHC staining of mouse kidney using 86321-1-RR (same clone as 86321-1-PBS)

    IHC staining of mouse kidney using 86321-1-RR (same clone as 86321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:80000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86321-1-PBS in a different storage buffer formulation.

  • WB analysis of rat kidney using 86321-1-RR (same clone as 86321-1-PBS)

    WB analysis of rat kidney using 86321-1-RR (same clone as 86321-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86321-1-PBS in a different storage buffer formulation.

  • WB analysis using 86321-1-RR (same clone as 86321-1-PBS)

    WB analysis using 86321-1-RR (same clone as 86321-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86321-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 86321-1-RR (same clone as 86321-1-PBS)

    IHC staining of human kidney using 86321-1-RR (same clone as 86321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86321-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 86321-1-RR (same clone as 86321-1-PBS)

    IHC staining of human kidney using 86321-1-RR (same clone as 86321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86321-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86321-1-RR

    Affinity and Kinetic Characterization of 86321-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86321-1-RR against Human ATP6V0A4 were performed. The affinity constant is 0.715 nM.

    {{ptg:ProductFeaturesInfo}}

ATP6V0A4 Recombinant monoclonal antibody, PBS Only

ATP6V0A4 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 86321-1-PBS

产品说明书

CloneNo. 250861E8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

A4, ATP6N1B, ATP6N2, RDRTA2, RTA1C

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86321-1-PBS targets ATP6V0A4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16095

Product name: Recombinant human ATP6V0A4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 190-375 aa of BC109305

Sequence: ERMASFERLLWRICRGNVYLKFSEMDAPLEDPVTKEEIQKNIFIIFYQGEQLRQKIKKICDGFRATVYPCPERAVERREMLESVNVRLEDLITVITQTESHRQRLLQEAAANWHSWLIKVQKMKAVYHILNMCNIDVTQQCVIAEIWFPVADATRIKRALEQGMELSGSSMAPIMTTVQSKTAPPTFNRTNKFTAG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal V0 subunit a4
别名 A4, ATP6N1B, ATP6N2, RDRTA2, RTA1C
计算分子量 840 aa, 96 kDa
观测分子量 85 kDa
GenBank蛋白编号BC109305
基因名称 ATP6V0A4
Gene ID (NCBI) 50617
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9HBG4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The ATP6V0A4 is a component of vacuolar-H+ATPase (V-ATPase) which is a multi-subunit enzyme that couples ATP hydrolysis to proton pumping across membranes. The V-ATPases are comprised of two major parts, the cytosolic V1 domain involved in ATP-binding and subsequent hydrolysis, and the membrane-associated V0 domain responsible for proton translocation. The V0 domain is composed of five subunits: a, c, c', c'' and d. The 'a' subunit of V0 domain has four isoforms : a1-a4. It has been found that mutations in ATP6V0A4(a4) are associated with distal renal tubular acidosis(dRTA) combined in some cases with progressive hearing loss leading to sensorineural deafness.