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验证数据展示

  • WB analysis using 21570-1-AP (same clone as 21570-1-PBS)

    WB analysis using 21570-1-AP (same clone as 21570-1-PBS)

    Mouse kidney lysates were subjected to SDS PAGE followed by western blot with 21570-1-AP (ATP6V0A4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21570-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 21570-1-AP (same clone as 21570-1-PBS)

    IHC staining of human kidney using 21570-1-AP (same clone as 21570-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21570-1-AP (ATP6V0A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21570-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse kidney using 21570-1-AP (same clone as 21570-1-PBS)

    IF Staining of mouse kidney using 21570-1-AP (same clone as 21570-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using ATP6V0A4 antibody (21570-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21570-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ATP6V0A4 Polyclonal antibody, PBS Only

ATP6V0A4 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 21570-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

A4, ATP6N1B, ATP6N2, RDRTA2, RTA1C

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

21570-1-PBS targets ATP6V0A4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag16095

Product name: Recombinant human ATP6V0A4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 190-375 aa of BC109305

Sequence: ERMASFERLLWRICRGNVYLKFSEMDAPLEDPVTKEEIQKNIFIIFYQGEQLRQKIKKICDGFRATVYPCPERAVERREMLESVNVRLEDLITVITQTESHRQRLLQEAAANWHSWLIKVQKMKAVYHILNMCNIDVTQQCVIAEIWFPVADATRIKRALEQGMELSGSSMAPIMTTVQSKTAPPTFNRTNKFTAG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal V0 subunit a4
别名 A4, ATP6N1B, ATP6N2, RDRTA2, RTA1C
计算分子量 840 aa, 96 kDa
观测分子量100 kDa
GenBank蛋白编号BC109305
基因名称 ATP6V0A4
Gene ID (NCBI) 50617
RRIDAB_2918072
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HBG4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The ATP6V0A4 is a component of vacuolar-H+ATPase (V-ATPase) which is a multi-subunit enzyme that couples ATP hydrolysis to proton pumping across membranes. The V-ATPases are comprised of two major parts, the cytosolic V1 domain involved in ATP-binding and subsequent hydrolysis, and the membrane-associated V0 domain responsible for proton translocation. The V0 domain is composed of five subunits: a, c, c', c'' and d. The 'a' subunit of V0 domain has four isoforms : a1-a4. It has been found that mutations in ATP6V0A4(a4) are associated with distal renal tubular acidosis(dRTA) combined in some cases with progressive hearing loss leading to sensorineural deafness. This antibody was generated against the internal region of human ATP6V0A4 and is predicted to detect the a4 isoform only.

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