- Featured Product
- KD/KO Validated
ATP6V0A4 Polyclonal antibody
ATP6V0A4 Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IHC, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
The immunogen of 21570-1-AP is ATP6V0A4 Fusion Protein expressed in E. coli.
| Tested Applications | IHC, WB,ELISA |
| Cited Applications | IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | ATP6V0A4 fusion protein Ag16095 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ATPase, H+ transporting, lysosomal V0 subunit a4 |
| Synonyms | A4, ATP6N1B, ATP6N2, ATP6V0A4, RDRTA2, RTA1C, RTADR, STV1, V ATPase 116 kDa isoform a4, VPH1, VPP2 |
| Calculated Molecular Weight | 840 aa, 96 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC109305 |
| Gene Symbol | ATP6V0A4 |
| Gene ID (NCBI) | 50617 |
| RRID | AB_2918072 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HBG4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The ATP6V0A4 is a component of vacuolar-H+ATPase (V-ATPase) which is a multi-subunit enzyme that couples ATP hydrolysis to proton pumping across membranes. The V-ATPases are comprised of two major parts, the cytosolic V1 domain involved in ATP-binding and subsequent hydrolysis, and the membrane-associated V0 domain responsible for proton translocation. The V0 domain is composed of five subunits: a, c, c', c'' and d. The 'a' subunit of V0 domain has four isoforms : a1-a4. It has been found that mutations in ATP6V0A4(a4) are associated with distal renal tubular acidosis(dRTA) combined in some cases with progressive hearing loss leading to sensorineural deafness. This antibody was generated against the internal region of human ATP6V0A4 and is predicted to detect the a4 isoform only.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP6V0A4 antibody 21570-1-AP | Download protocol |
| IHC protocol for ATP6V0A4 antibody 21570-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncol Lett Identification of ATP6V0A4 as a potential biomarker in renal cell carcinoma using integrated bioinformatics analysis |