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验证数据展示

  • WB analysis using 16335-1-AP (same clone as 16335-1-PBS)

    WB analysis using 16335-1-AP (same clone as 16335-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16335-1-AP (ATP5S antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16335-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 16335-1-AP (same clone as 16335-1-PBS)

    IF Staining of U2OS using 16335-1-AP (same clone as 16335-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using ATP5S antibody (16335-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16335-1-PBS in a different storage buffer formulation.

ATP5S Polyclonal antibody, PBS Only

ATP5S Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 16335-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

ATP synthase-coupling factor B, ATPW, Distal membrane arm assembly complex 2-like protein, DMAC2L, FB

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16335-1-PBS targets ATP5S in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9486

Product name: Recombinant human ATP5S protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-127 aa of BC011549

Sequence: MCCAVSEQRLTCADQMMLFGKISQQLCGVKKLPWSCDSRYFWGWLNAVFNKVDYDRIRDVGPDRAASEWLLRCGAMVRYHGQERWQKDYNHLPTGPLDKYKIQAIDATDSCIMSIGFDHMETSNICC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit s (factor B)
别名 ATP synthase-coupling factor B, ATPW, Distal membrane arm assembly complex 2-like protein, DMAC2L, FB
计算分子量127aa,15 kDa; 215aa,25 kDa
观测分子量 23 kDa
GenBank蛋白编号BC011549
基因名称 ATP5S
Gene ID (NCBI) 27109
RRIDAB_3741884
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99766
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DMAC2L also known as ATP5S, belongs to the ATP synthase family, which is a group of enzymes that play a crucial role in cellular energy production. ATP synthase is the central enzyme in oxidative phosphorylation, responsible for the production of most of the ATP in mammalian organisms. ATP5S is the S subunit (also known as factor B) of mitochondrial FO complex of ATP synthase. It is an essential component of the H+ channel of the FO complex (PMID:7706317, 6143319).

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