验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, K-562 cells, human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
16335-1-AP targets ATP5S in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | ATP5S fusion protein Ag9486 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATP synthase, H+ transporting, mitochondrial F0 complex, subunit s (factor B) |
| 别名 | FB, DMAC2L, Distal membrane arm assembly complex 2-like protein, ATPW, ATP synthase-coupling factor B |
| 计算分子量 | 127aa,15 kDa; 215aa,25 kDa |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | BC011549 |
| 基因名称 | ATP5S |
| Gene ID (NCBI) | 27109 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99766 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DMAC2L also known as ATP5S, belongs to the ATP synthase family, which is a group of enzymes that play a crucial role in cellular energy production. ATP synthase is the central enzyme in oxidative phosphorylation, responsible for the production of most of the ATP in mammalian organisms. ATP5S is the S subunit (also known as factor B) of mitochondrial FO complex of ATP synthase. It is an essential component of the H+ channel of the FO complex (PMID:7706317, 6143319).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP5S antibody 16335-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |