验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, Jurkat cells, Ramos cells, SK-BR-3 cells, PANC-1 cells, mouse liver tissue, rat liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86174-2-RR targets ATP5H in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | ATP5H fusion protein Ag11429 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d |
| 别名 | ATP synthase peripheral stalk subunit d, ATP5, ATP5JD, ATP5PD, ATPQ |
| 计算分子量 | 137 aa, 16 kDa |
| 观测分子量 | 19-22 kDa |
| GenBank蛋白编号 | BC032245 |
| 基因名称 | ATP5H |
| Gene ID (NCBI) | 10476 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O75947 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5H gene encodes ATP synthase subunit d of the Fo complex.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP5H antibody 86174-2-RR | Download protocol |
| IF protocol for ATP5H antibody 86174-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |