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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 86174-2-RR (same clone as 86174-2-PBS)

    WB analysis using 86174-2-RR (same clone as 86174-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86174-2-RR (ATP5H antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86174-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 86174-2-RR (same clone as 86174-2-PBS)

    IF Staining of HepG2 using 86174-2-RR (same clone as 86174-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATP5H antibody (86174-2-RR, Clone: 250751G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86174-2-PBS in a different storage buffer formulation.

ATP5H Recombinant antibody, PBS Only

ATP5H Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86174-2-PBS

产品说明书

CloneNo. 250751G4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

ATP synthase peripheral stalk subunit d, ATP5, ATP5JD, ATP5PD, ATPQ

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86174-2-PBS targets ATP5H in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ATP5H fusion protein Ag11429 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
别名 ATP synthase peripheral stalk subunit d, ATP5, ATP5JD, ATP5PD, ATPQ
计算分子量 137 aa, 16 kDa
观测分子量 19-22 kDa
GenBank蛋白编号BC032245
基因名称 ATP5H
Gene ID (NCBI) 10476
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75947
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5H gene encodes ATP synthase subunit d of the Fo complex.