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验证数据展示

  • WB analysis using 15999-1-AP (same clone as 15999-1-PBS)

    WB analysis using 15999-1-AP (same clone as 15999-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15999-1-AP (ATP5F1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 15999-1-AP (same clone as 15999-1-PBS)

    WB analysis of HeLa using 15999-1-AP (same clone as 15999-1-PBS)

    WB result of ATP5F1 antibody (15999-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP5F1 transfected HeLa cells. This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293T using 15999-1-AP (same clone as 15999-1-PBS)

    IP experiment of HEK-293T using 15999-1-AP (same clone as 15999-1-PBS)

    IP result of anti-ATP5F1 (IP:15999-1-AP, 4ug; Detection:15999-1-AP 1:2000) with HEK-293T cells lysate 1600 ug. This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 15999-1-AP (same clone as 15999-1-PBS)

    IHC staining of human liver using 15999-1-AP (same clone as 15999-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15999-1-AP (ATP5F1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 15999-1-AP (same clone as 15999-1-PBS)

    IHC staining of human liver using 15999-1-AP (same clone as 15999-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15999-1-AP (ATP5F1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 15999-1-AP (same clone as 15999-1-PBS)

    FC experiment of HeLa using 15999-1-AP (same clone as 15999-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATP5F1 (15999-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 15999-1-AP (same clone as 15999-1-PBS)

    IF Staining of HeLa using 15999-1-AP (same clone as 15999-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATP5F1 antibody (15999-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ATP5F1 Polyclonal antibody, PBS Only

ATP5F1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 15999-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

ATP synthase F(0) complex subunit B1, mitochondrial, ATP synthase peripheral stalk-membrane subunit b, ATP synthase proton-transporting mitochondrial F(0) complex subunit B1, ATP synthase subunit b, ATP5F 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15999-1-PBS targets ATP5F1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag8571

Product name: Recombinant human ATP5F1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-256 aa of BC005366

Sequence: MLSRVVLSAAATAAPSLKNAAFLGPGVLQATRTFHTGQPHLVPVPPLPEYGGKVRYGLIPEEFFQFLYPKTGVTGPYVLGTGLILYALSKEIYVISAETFTALSVLGVMVYGIKKYGPFVADFADKLNEQKLAQLEEAKQASIQHIQNAIDTEKSQQALVQKRHYLFDVQRNNIAMALEVTYRERLYRVYKEVKNRLDYHISVQNMMRRKEQEHMINWVEKHVVQSISTQQEKETIAKCIADLKLLAKKAQAQPVM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
别名 ATP synthase F(0) complex subunit B1, mitochondrial, ATP synthase peripheral stalk-membrane subunit b, ATP synthase proton-transporting mitochondrial F(0) complex subunit B1, ATP synthase subunit b, ATP5F 1
计算分子量 256 aa, 29 kDa
观测分子量 25-30 kDa
GenBank蛋白编号BC005366
基因名称 ATP5F1
Gene ID (NCBI) 515
RRIDAB_2258817
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP24539
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP5F1(ATP synthase subunit b) belongs to the eukaryotic ATPase B chain family. The ATP5F1 gene encodes subunit B of the mitochondrial ATP synthase Fo unit, which contains 214-amino acid with a 42-amino acid import signal(PMID:1831354). Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.

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