验证数据展示

WB analysis using 15999-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15999-1-AP (ATP5F1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15999-1-AP
WB result of ATP5F1 antibody (15999-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP5F1 transfected HeLa cells.
IP experiment of HEK-293T using 15999-1-AP
IP result of anti-ATP5F1 (IP:15999-1-AP, 4ug; Detection:15999-1-AP 1:2000) with HEK-293T cells lysate 1600 ug.
IHC staining of human liver using 15999-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15999-1-AP (ATP5F1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 15999-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15999-1-AP (ATP5F1 Antibody) at dilution of 1:200 (under 40x lens).
FC experiment of HeLa using 15999-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATP5F1 (15999-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
IF Staining of HeLa using 15999-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATP5F1 antibody (15999-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO Validated

ATP5F1 Polyclonal antibody

ATP5F1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15999-1-AP

产品说明书

引用文献(29)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

别名

ATP synthase F(0) complex subunit B1, mitochondrial, ATP synthase peripheral stalk-membrane subunit b, ATP synthase proton-transporting mitochondrial F(0) complex subunit B1, ATP synthase subunit b, ATP5F 1

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (29)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	mouse heart tissue, HeLa cells, mouse skeletal muscle tissue, mouse liver tissue
Positive IP detected in	HEK-293T cells
Positive IHC detected in	human liver tissue, human brain tissue, human heart tissue, human kidney tissue, human skin tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 25 publications below
IHC	See 2 publications below
IF	See 2 publications below
CoIP	See 1 publications below

产品信息

15999-1-AP targets ATP5F1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, CoIP
经测试反应性	human, mouse
文献引用反应性	human, mouse, rat
免疫原	CatNo: Ag8571 Product name: Recombinant human ATP5F1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-256 aa of BC005366 Sequence: MLSRVVLSAAATAAPSLKNAAFLGPGVLQATRTFHTGQPHLVPVPPLPEYGGKVRYGLIPEEFFQFLYPKTGVTGPYVLGTGLILYALSKEIYVISAETFTALSVLGVMVYGIKKYGPFVADFADKLNEQKLAQLEEAKQASIQHIQNAIDTEKSQQALVQKRHYLFDVQRNNIAMALEVTYRERLYRVYKEVKNRLDYHISVQNMMRRKEQEHMINWVEKHVVQSISTQQEKETIAKCIADLKLLAKKAQAQPVM 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
别名	ATP synthase F(0) complex subunit B1, mitochondrial, ATP synthase peripheral stalk-membrane subunit b, ATP synthase proton-transporting mitochondrial F(0) complex subunit B1, ATP synthase subunit b, ATP5F 1
计算分子量	256 aa, 29 kDa
观测分子量	25-30 kDa
GenBank蛋白编号	BC005366
基因名称	ATP5F1
Gene ID (NCBI)	515
RRID	AB_2258817
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P24539
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

ATP5F1(ATP synthase subunit b) belongs to the eukaryotic ATPase B chain family. The ATP5F1 gene encodes subunit B of the mitochondrial ATP synthase Fo unit, which contains 214-amino acid with a 42-amino acid import signal(PMID:1831354). Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.

实验方案

Product Specific Protocols
FC protocol for ATP5F1 antibody 15999-1-AP	Download protocol
IF protocol for ATP5F1 antibody 15999-1-AP	Download protocol
IHC protocol for ATP5F1 antibody 15999-1-AP	Download protocol
IP protocol for ATP5F1 antibody 15999-1-AP	Download protocol
WB protocol for ATP5F1 antibody 15999-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IF	Adv Mater Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration Authors - Zhuo Li View Article
rat	WB	Proc Natl Acad Sci U S A Evaluation of intramitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation. Authors - Hidetaka Kioka View Article
mouse	WB,IHC	Part Fibre Toxicol Transcriptomics of single dose and repeated carbon black and ozone inhalation co-exposure highlight progressive pulmonary mitochondrial dysfunction. Authors - Quincy A Hathaway View Article
human	WB	Free Radic Biol Med Mitochondrial and glucose metabolic dysfunctions in granulosa cells induce impaired oocytes of polycystic ovary syndrome through Sirtuin 3. Authors - Qing Zhang View Article
mouse	WB	J Neurosci A Mutation in Hnrnph1 That Decreases Methamphetamine-Induced Reinforcement, Reward, and Dopamine Release and Increases Synaptosomal hnRNP H and Mitochondrial Proteins. Authors - Qiu T Ruan View Article
rat	WB	Cell Death Dis Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway. Authors - Yuehong Wang View Article

验证数据展示

WB analysis using 15999-1-AP

WB analysis of HeLa using 15999-1-AP

IP experiment of HEK-293T using 15999-1-AP

IHC staining of human liver using 15999-1-AP

IHC staining of human liver using 15999-1-AP

FC experiment of HeLa using 15999-1-AP

IF Staining of HeLa using 15999-1-AP

ATP5F1 Polyclonal antibody

ATP5F1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration

Evaluation of intramitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation.

Transcriptomics of single dose and repeated carbon black and ozone inhalation co-exposure highlight progressive pulmonary mitochondrial dysfunction.

Mitochondrial and glucose metabolic dysfunctions in granulosa cells induce impaired oocytes of polycystic ovary syndrome through Sirtuin 3.

A Mutation in Hnrnph1 That Decreases Methamphetamine-Induced Reinforcement, Reward, and Dopamine Release and Increases Synaptosomal hnRNP H and Mitochondrial Proteins.

Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.

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