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验证数据展示

  • WB analysis of human heart using 60284-1-Ig (same clone as 60284-1-PBS)

    WB analysis of human heart using 60284-1-Ig (same clone as 60284-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 60284-1-Ig (ATP5C1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60284-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60284-1-Ig (same clone as 60284-1-PBS)

    IHC staining of human liver cancer using 60284-1-Ig (same clone as 60284-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60284-1-Ig (ATP5C1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60284-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60284-1-Ig (same clone as 60284-1-PBS)

    IHC staining of human liver cancer using 60284-1-Ig (same clone as 60284-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60284-1-Ig (ATP5C1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60284-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60284-1-Ig (same clone as 60284-1-PBS)

    IF Staining of HepG2 using 60284-1-Ig (same clone as 60284-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60284-1-Ig (ATP5C1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60284-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 60284-1-Ig (same clone as 60284-1-PBS)

    FC experiment of HeLa using 60284-1-Ig (same clone as 60284-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATP5C1 (60284-1-Ig, Clone:1B5A2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60284-1-PBS in a different storage buffer formulation.

ATP5C1 Monoclonal antibody, PBS Only

ATP5C1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 60284-1-PBS

产品说明书

CloneNo. 1B5A2

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

F-ATPase gamma subunit, ATP5F1C, ATP5CL1, ATP5C, ATP synthase F1 subunit gamma

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60284-1-PBS targets ATP5C1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 ATP5C1 fusion protein Ag1343 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1
别名 F-ATPase gamma subunit, ATP5F1C, ATP5CL1, ATP5C, ATP synthase F1 subunit gamma
计算分子量 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC000931
基因名称 ATP5C1
Gene ID (NCBI) 509
RRIDAB_2881402
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP36542
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP5C1(ATP synthase subunit gamma, mitochondrial) is also named as ATP5C, ATP5CL1 and belongs to the ATPase gamma chain family. Some scientists reported the complete sequence of the gene for the human ATP synthase gamma subunit and described tissue-specific isoforms of the subunit generated by alternative splicing of exon 9. The liver (L) isoform differed from the heart (H) isoform by the addition of a single amino acid (asp273) at the C terminus. (PMID:8227057).