验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84213-5 targets ATP2A1 in IF-P applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag17944 Product name: Recombinant human ATP2A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 561-635 aa of BC037354 Sequence: RCNYVRVGTTRVPLTGPVKEKIMAVIKEWGTGRDTLRCLALATRDTPPKREEMVLDDSARFLEYETDLTFVGVVG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ATPase, Ca++ transporting, cardiac muscle, fast twitch 1 |
| 别名 | 241500D12, ATP2A, ATP2A 1, Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform, EC:7.2.2.10 |
| 计算分子量 | 1001 aa, 110 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC037354 |
| 基因名称 | ATP2A1 |
| Gene ID (NCBI) | 487 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O14983 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP2A1 also known as SERCA1, encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen and is involved in muscular excitation and contraction. Mutations in ATP2A1 cause some autosomal recessive forms of Brody disease(PMID: 23911890, 10914677).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ATP2A1 antibody CL488-84213-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
