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验证数据展示

  • WB analysis using 83608-5-RR

    WB analysis using 83608-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83608-5-RR (ATM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 83608-5-RR

    IHC staining of mouse brain using 83608-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83608-5-RR (ATM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATM antibody included
  • IF Staining of MCF-7 using 83608-5-RR

    IF Staining of MCF-7 using 83608-5-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ATM antibody (83608-5-RR, Clone: 240560F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 83608-5-RR

    IF Staining of HepG2 using 83608-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATM antibody (83608-5-RR, Clone: 240560F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 83608-5-RR

    IF Staining of HeLa using 83608-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATM antibody (83608-5-RR, Clone: 240560F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of MCF-7 using 83608-5-RR

    FC experiment of MCF-7 using 83608-5-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ATM Recombinant antibody (83608-5-RR, Clone:240560F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83608-5-RR

    Affinity and Kinetic Characterization of 83608-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83608-5-RR against Human ATM were performed. The affinity constant is 0.318 nM.

ATM Recombinant antibody

ATM Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83608-5-RR

产品说明书

CloneNo. 240560F5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

240560F5, A T mutated, A-T mutated, EC:2.7.11.1, Serine-protein kinase ATM

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, Raji cells, MCF-7 cells, A549 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells, MCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy ATM Ready-To-Use IHC Kit. ATM primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83608-5-RR targets ATM in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 ATM fusion protein Ag25920 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ataxia telangiectasia mutated
别名 240560F5, A T mutated, A-T mutated, EC:2.7.11.1, Serine-protein kinase ATM
观测分子量 310-350 kDa
GenBank蛋白编号BC137169
基因名称 ATM
Gene ID (NCBI) 472
RRIDAB_3671222
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13315
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.

实验方案

Product Specific Protocols
WB protocol for ATM antibody 83608-5-RRDownload protocol
IHC protocol for ATM antibody 83608-5-RRDownload protocol
IF protocol for ATM antibody 83608-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols