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验证数据展示

  • WB analysis using 83608-5-RR (same clone as 83608-5-PBS)

    WB analysis using 83608-5-RR (same clone as 83608-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83608-5-RR (ATM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 83608-5-RR (same clone as 83608-5-PBS)

    WB analysis of A549 using 83608-5-RR (same clone as 83608-5-PBS)

    WB result of ATM antibody (83608-5-RR; 1:3000; room temperature for 1.5 hours) with wild-type and sg-ATM transfected A549 cells. This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83608-5-RR (same clone as 83608-5-PBS)

    IHC staining of mouse brain using 83608-5-RR (same clone as 83608-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83608-5-RR (ATM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATM antibody included
  • IF Staining of MCF-7 using 83608-5-RR (same clone as 83608-5-PBS)

    IF Staining of MCF-7 using 83608-5-RR (same clone as 83608-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ATM antibody (83608-5-RR, Clone: 240560F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83608-5-RR (same clone as 83608-5-PBS)

    IF Staining of HepG2 using 83608-5-RR (same clone as 83608-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATM antibody (83608-5-RR, Clone: 240560F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83608-5-RR (same clone as 83608-5-PBS)

    IF Staining of HeLa using 83608-5-RR (same clone as 83608-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATM antibody (83608-5-RR, Clone: 240560F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83608-5-RR (same clone as 83608-5-PBS)

    FC experiment of MCF-7 using 83608-5-RR (same clone as 83608-5-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ATM Recombinant antibody (83608-5-RR, Clone:240560F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83608-5-RR

    Affinity and Kinetic Characterization of 83608-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83608-5-RR against Human ATM were performed. The affinity constant is 0.318 nM.

  • Featured Product
  • KD/KO验证

ATM Recombinant monoclonal antibody, PBS Only

ATM Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83608-5-PBS

产品说明书

CloneNo. 240560F5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

240560F5, A T mutated, A-T mutated, EC:2.7.11.1, Serine-protein kinase ATM

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ATM Ready-To-Use IHC Kit. ATM primary antibody included.

产品信息

83608-5-PBS targets ATM in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag25920

Product name: Recombinant human ATM protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1059-1361 aa of BC137169

Sequence: SSEFENKQALLKRAKEEVGLLREHKIQTNRYTVKVQRELELDELALRALKEDRKRFLCKAVENYINCLLSGEEHDMWVFRLCSLWLENSGVSEVNGMMKRDGMKIPTYKFLPLMYQLAARMGTKMMGGLGFHEVLNNLISRISMDHPHHTLFIILALANANRDEFLTKPEVARRSRITKNVPKQSSQLDEDRTEAANRIICTIRSRRPQMVRSVEALCDAYIILANLDATQWKTQRKGINIPADQPITKLKNLEDVVVPTMEIKVDHTGEYGNLVTIQSFKAEFRLAGGVNLPKIIDCVGSDG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ataxia telangiectasia mutated
别名 240560F5, A T mutated, A-T mutated, EC:2.7.11.1, Serine-protein kinase ATM
观测分子量 310-350 kDa
GenBank蛋白编号BC137169
基因名称 ATM
Gene ID (NCBI) 472
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ13315
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.

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