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验证数据展示

  • WB analysis using 84578-3-RR

    WB analysis using 84578-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84578-3-RR (ATL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84578-3-RR

    IF Staining of HeLa using 84578-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATL3 antibody (84578-3-RR, Clone: 241937G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84578-3-RR

    Affinity and Kinetic Characterization of 84578-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84578-3-RR against Human ATL3 were performed. The affinity constant is 1.14 nM.

ATL3 Recombinant monoclonal antibody

ATL3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84578-3-RR

产品说明书

CloneNo. 241937G3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

241937G3, Atlastin 3, Atlastin-3, EC:3.6.5.-

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, HEK-293 cells, PC-3 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84578-3-RR targets ATL3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10248

Product name: Recombinant human ATL3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-351 aa of BC077727

Sequence: MLSPQRVAAAASRGADDAMESSKPGPVQVVLVQKDQHSFELDEKALASILLQDHIRDLDVVVVSVAGAFRKGKSFILDFMLRYLYSQKESGHSNWLGDPEEPLTGFSWRGGSDPETTGIQIWSEVFTVEKPGGKKVAVVLMDTQGAFDSQSTVKDCATIFALSTMTSSVQIYNLSQNIQEDDLQQLQLFTEYGRLAMDEIFQKPFQTLMFLVRDWSFPYEYSYGLQGGMAFLDKRLQVKEHQHEEIQNVRNHIHSCFSDVTCFLLPHPGLQVATSPDFDGKLKDIAGEFKEQLQALIPYVLNPSKLMEKEINGSKVTCRGLLEYFKAYIKIYQGEDLPHPKSMLQATAEAN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 atlastin GTPase 3
别名 241937G3, Atlastin 3, Atlastin-3, EC:3.6.5.-
计算分子量 541 aa, 61 kDa
观测分子量 58-61 kDa
GenBank蛋白编号BC077727
基因名称 ATL3
Gene ID (NCBI) 25923
RRIDAB_3672078
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ6DD88
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATL3, belonging to a class of dynamin-like GTPase, was reported to mediate ER fusion. ATL3 is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. ATL3 functions as a receptor for ER-phagy, promoting tubular ER degradation upon starvation. Mutations in this gene may be associated with hereditary sensory neuropathy type IF.

实验方案

Product Specific Protocols
IF protocol for ATL3 antibody 84578-3-RRDownload protocol
WB protocol for ATL3 antibody 84578-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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