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验证数据展示

  • WB analysis using 84578-3-RR (same clone as 84578-3-PBS)

    WB analysis using 84578-3-RR (same clone as 84578-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84578-3-RR (ATL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84578-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84578-3-RR (same clone as 84578-3-PBS)

    IF Staining of HeLa using 84578-3-RR (same clone as 84578-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATL3 antibody (84578-3-RR, Clone: 241937G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84578-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84578-3-RR

    Affinity and Kinetic Characterization of 84578-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84578-3-RR against Human ATL3 were performed. The affinity constant is 1.14 nM.

ATL3 Recombinant monoclonal antibody, PBS Only

ATL3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84578-3-PBS

产品说明书

CloneNo. 241937G3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

241937G3, Atlastin 3, Atlastin-3, EC:3.6.5.-

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84578-3-PBS targets ATL3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10248

Product name: Recombinant human ATL3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-351 aa of BC077727

Sequence: MLSPQRVAAAASRGADDAMESSKPGPVQVVLVQKDQHSFELDEKALASILLQDHIRDLDVVVVSVAGAFRKGKSFILDFMLRYLYSQKESGHSNWLGDPEEPLTGFSWRGGSDPETTGIQIWSEVFTVEKPGGKKVAVVLMDTQGAFDSQSTVKDCATIFALSTMTSSVQIYNLSQNIQEDDLQQLQLFTEYGRLAMDEIFQKPFQTLMFLVRDWSFPYEYSYGLQGGMAFLDKRLQVKEHQHEEIQNVRNHIHSCFSDVTCFLLPHPGLQVATSPDFDGKLKDIAGEFKEQLQALIPYVLNPSKLMEKEINGSKVTCRGLLEYFKAYIKIYQGEDLPHPKSMLQATAEAN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 atlastin GTPase 3
别名 241937G3, Atlastin 3, Atlastin-3, EC:3.6.5.-
计算分子量 541 aa, 61 kDa
观测分子量 58-61 kDa
GenBank蛋白编号BC077727
基因名称 ATL3
Gene ID (NCBI) 25923
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ6DD88
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATL3, belonging to a class of dynamin-like GTPase, was reported to mediate ER fusion. ATL3 is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. ATL3 functions as a receptor for ER-phagy, promoting tubular ER degradation upon starvation. Mutations in this gene may be associated with hereditary sensory neuropathy type IF.

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