• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

ATG5 Recombinant antibody, PBS Only

ATG5 Recombinant Antibody for WB,IP,Indirect ELISA,IHC

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse , rat

Applications

WB,IP,Indirect ELISA,IHC

Conjugate

Unconjugated

CloneNo.

5M5

Cat No : 81803-1-PBS

Print datasheet

Synonyms

APG5, APG5 LIKE, APG5L, Apoptosis specific protein, ASP, ATG5, Autophagy protein 5, hAPG5

验证数据展示

  • WB analysis using 81803-1-RR (same clone as 81803-1-PBS)

    WB analysis using 81803-1-RR (same clone as 81803-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81803-1-RR (ATG5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81803-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 81803-1-RR (same clone as 81803-1-PBS)

    WB analysis of HeLa using 81803-1-RR (same clone as 81803-1-PBS)

    WB result of ATG5 antibody (81803-1-RR; 1:6000; room temperature for 1.5 hours) with wild-type and ATG5 knockout HeLa cells. This data was developed using the same antibody clone with 81803-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 81803-1-RR (same clone as 81803-1-PBS)

    IP experiment of HeLa using 81803-1-RR (same clone as 81803-1-PBS)

    IP result of anti-ATG5 (IP:81803-1-RR, 4ug; Detection:81803-1-RR 1:2000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 81803-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 81803-1-RR (same clone as 81803-1-PBS)

    IHC staining of human ovary tumor using 81803-1-RR (same clone as 81803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81803-1-RR (ATG5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81803-1-PBS in a different storage buffer formulation.

产品信息

81803-1-PBS targets ATG5 in WB, IP, IHC, Indirect ELISA applications and shows reactivity with Human, mouse , rat samples.

Tested Applications WB,IP,Indirect ELISA,IHC
Tested Reactivity Human, mouse , rat
Immunogen ATG5 fusion protein Ag0214 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATG5 autophagy related 5 homolog (S. cerevisiae)
Synonyms APG5, APG5 LIKE, APG5L, Apoptosis specific protein, ASP, ATG5, Autophagy protein 5, hAPG5
Calculated Molecular Weight 32 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC002699
Gene Symbol ATG5
Gene ID (NCBI) 9474
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H1Y0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band.