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验证数据展示

  • WB analysis using 81803-1-RR (same clone as 81803-1-PBS)

    WB analysis using 81803-1-RR (same clone as 81803-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81803-1-RR (ATG5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81803-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 81803-1-RR (same clone as 81803-1-PBS)

    WB analysis of HeLa using 81803-1-RR (same clone as 81803-1-PBS)

    WB result of ATG5 antibody (81803-1-RR; 1:6000; room temperature for 1.5 hours) with wild-type and ATG5 knockout HeLa cells. This data was developed using the same antibody clone with 81803-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 81803-1-RR (same clone as 81803-1-PBS)

    IP experiment of HeLa using 81803-1-RR (same clone as 81803-1-PBS)

    IP result of anti-ATG5 (IP:81803-1-RR, 4ug; Detection:81803-1-RR 1:2000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 81803-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 81803-1-RR (same clone as 81803-1-PBS)

    IHC staining of human ovary tumor using 81803-1-RR (same clone as 81803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81803-1-RR (ATG5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81803-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81803-1-RR

    Affinity and Kinetic Characterization of 81803-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81803-1-RR against Human ATG5 were performed. The affinity constant is 42.4 nM.

  • Featured Product
  • KD/KO验证

ATG5 Recombinant antibody, PBS Only

ATG5 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 81803-1-PBS

产品说明书

CloneNo. 5M5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

Apoptosis specific protein, APG5-like, APG5L, APG5 LIKE, APG5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

81803-1-PBS targets ATG5 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ATG5 fusion protein Ag0214 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATG5 autophagy related 5 homolog (S. cerevisiae)
别名 Apoptosis specific protein, APG5-like, APG5L, APG5 LIKE, APG5
计算分子量 32 kDa
观测分子量50-55 kDa
GenBank蛋白编号BC002699
基因名称 ATG5
Gene ID (NCBI) 9474
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H1Y0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band.