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ATG16L1 Monoclonal antibody, PBS Only

ATG16L1 Monoclonal Antibody for WB,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,IF,ELISA

Conjugate

Unconjugated

CloneNo.

1G5G7

Cat No : 67943-1-PBS

Print datasheet

Synonyms

APG16 like 1, APG16L, ATG16L, ATG16L1, Autophagy related protein 16 1, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, WDR30

验证数据展示

  • WB analysis using 67943-1-Ig (same clone as 67943-1-PBS)

    WB analysis using 67943-1-Ig (same clone as 67943-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67943-1-Ig (ATG16L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67943-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67943-1-Ig (same clone as 67943-1-PBS)

    IF Staining of HepG2 using 67943-1-Ig (same clone as 67943-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATG16L1 antibody (67943-1-Ig, Clone: 1G5G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67943-1-PBS in a different storage buffer formulation.

产品信息

67943-1-PBS targets ATG16L1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,IF,ELISA
Tested Reactivity Human, mouse, rat
Immunogen ATG16L1 fusion protein Ag14881 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATG16 autophagy related 16-like 1 (S. cerevisiae)
Synonyms APG16 like 1, APG16L, ATG16L, ATG16L1, Autophagy related protein 16 1, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, WDR30
Calculated Molecular Weight 607 aa, 68 kDa
Observed Molecular Weight68-75 kDa
GenBank Accession NumberBC000061
Gene Symbol ATG16L1
Gene ID (NCBI) 55054
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ676U5
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human ATG16L1 is a 607 amino acid protein (~68 kDa) comprising three major domains: the N‐terminal ATG5 binding domain (ATG5‐BD), the central coiled‐coil domain (CCD) and a predicted C‐terminal WD40‐domain. ATG16L1α and β (Atg16L1α, 63 kDa; and Atg16L1β, 71 kDa) are the major isoforms expressed in intestinal epithelium and macrophages , and all isoforms encode exon 9, which contains Thr 300. Atg16L1 mediates the cellular degradative process of autophagy and is considered a critical regulator of inflammation based on its genetic association with inflammatory bowel disease. ATG16L1 has been implicated in Crohn's disease. (PMID: 24553140, PMID: 22740627,PMID: 28685931)