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验证数据展示

  • WB analysis using 30505-1-AP

    WB analysis using 30505-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30505-1-AP (ATG12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30505-1-AP

    WB analysis using 30505-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30505-1-AP (ATG12 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 30505-1-AP

    IHC staining of human colon cancer using 30505-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30505-1-AP (ATG12 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-3 using 30505-1-AP

    IF Staining of PC-3 using 30505-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ATG12 antibody (30505-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ATG12 Polyclonal antibody

ATG12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30505-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

APG12, APG12 like, APG12L, APG12-like, Autophagy-related protein 12

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, COLO 320 cells, NIH/3T3 cells, PC-3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30505-1-AP targets ATG12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32268

Product name: Recombinant human ATG12 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 48-187 aa of BC012266

Sequence: MAEEPQSVLQLPTSIAAGGEGLTDVSPETTTPEPPSSAAVSPGTEEPAGDTKKKIDILLKAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLKLVASEQLFIYVNQSFAPSPDQEVGTLYECFGSDGKLVLHYCKSQAWG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATG12 autophagy related 12 homolog (S. cerevisiae)
别名 APG12, APG12 like, APG12L, APG12-like, Autophagy-related protein 12
计算分子量 15 kDa
观测分子量20 kDa, 50-60 kDa
GenBank蛋白编号BC012266
基因名称 ATG12
Gene ID (NCBI) 9140
RRIDAB_3086340
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94817
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.

实验方案

Product Specific Protocols
IF protocol for ATG12 antibody 30505-1-APDownload protocol
IHC protocol for ATG12 antibody 30505-1-APDownload protocol
WB protocol for ATG12 antibody 30505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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