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验证数据展示

  • WB analysis using 87421-1-RR

    WB analysis using 87421-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87421-1-RR (ATF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 87421-1-RR

    WB analysis of A549 using 87421-1-RR

    WB result of ATF1 antibody (87421-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF1 transfected A549 cells.

  • IF Staining of HeLa using 87421-1-RR

    IF Staining of HeLa using 87421-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATF1 antibody (87421-1-RR, Clone: 252816B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of NIH/3T3 using 87421-1-RR

    IF Staining of NIH/3T3 using 87421-1-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using ATF1 antibody (87421-1-RR, Clone: 252816B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • KD/KO Validated

ATF1 Recombinant monoclonal antibody

ATF1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87421-1-RR

产品说明书

CloneNo. 252816B3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

cAMP-dependent transcription factor ATF-1, Cyclic AMP-dependent transcription factor ATF-1, TREB36

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, HepG2 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells, NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87421-1-RR targets ATF1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag2548

Product name: Recombinant human ATF1 protein

Source: e coli.-derived, T-GST

Tag: GST

Domain: 1-271 aa of BC029619

Sequence: MEDSHKSTTSETAPQPGSAVQGAHISHIAQQVSSLSESEESQDSSDSIGSSQKAHGILARRPSYRKILKDLSSEDTRGRKGDGENSGVSAAVTSMSVPTPIYQTSSGQYIAIAPNGALQLASPGTDGVQGLQTLTMTNSGSTQQGTTILQYAQTSDGQQILVPSNQVVVQTASGDMQTYQIRTTPSATSLPQTVVMTSPVTLTSQTTKTDDPQLKREIRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLYSNKSV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 activating transcription factor 1
别名 cAMP-dependent transcription factor ATF-1, Cyclic AMP-dependent transcription factor ATF-1, TREB36
计算分子量 271 aa, 29 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC029619
基因名称 ATF1
Gene ID (NCBI) 466
RRIDAB_3745533
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP18846
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TFs play a vital role in maintaining cell homeostasis by combining DNA regulatory sequences to regulate gene transcription rates. Activating transcription factor 1 (ATF1) is a member of the ATF/CREB family of transcription factors (TFs), specifically interacting with the consensus ATF/CRE site 'TGACGTCA'(PMID: 28032861). ATF1 transcriptionally regulates the expressions of target genes to mediate various cellular processes, and ATF1 effects the progression of tumors (including lung cancer, colorectal cancer,and nasopharyngeal carcinoma).

实验方案

Product Specific Protocols
IF protocol for ATF1 antibody 87421-1-RRDownload protocol
WB protocol for ATF1 antibody 87421-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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