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验证数据展示

  • WB analysis using 87421-1-RR (same clone as 87421-1-PBS)

    WB analysis using 87421-1-RR (same clone as 87421-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87421-1-RR (ATF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87421-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 87421-1-RR (same clone as 87421-1-PBS)

    WB analysis of A549 using 87421-1-RR (same clone as 87421-1-PBS)

    WB result of ATF1 antibody (87421-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF1 transfected A549 cells. This data was developed using the same antibody clone with 87421-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 87421-1-RR (same clone as 87421-1-PBS)

    IF Staining of HeLa using 87421-1-RR (same clone as 87421-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATF1 antibody (87421-1-RR, Clone: 252816B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87421-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 87421-1-RR (same clone as 87421-1-PBS)

    IF Staining of NIH/3T3 using 87421-1-RR (same clone as 87421-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using ATF1 antibody (87421-1-RR, Clone: 252816B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87421-1-PBS in a different storage buffer formulation.

ATF1 Recombinant monoclonal antibody, PBS Only

ATF1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87421-1-PBS

产品说明书

CloneNo. 252816B3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

cAMP-dependent transcription factor ATF-1, Cyclic AMP-dependent transcription factor ATF-1, TREB36

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87421-1-PBS targets ATF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag2548

Product name: Recombinant human ATF1 protein

Source: e coli.-derived, T-GST

Tag: GST

Domain: 1-271 aa of BC029619

Sequence: MEDSHKSTTSETAPQPGSAVQGAHISHIAQQVSSLSESEESQDSSDSIGSSQKAHGILARRPSYRKILKDLSSEDTRGRKGDGENSGVSAAVTSMSVPTPIYQTSSGQYIAIAPNGALQLASPGTDGVQGLQTLTMTNSGSTQQGTTILQYAQTSDGQQILVPSNQVVVQTASGDMQTYQIRTTPSATSLPQTVVMTSPVTLTSQTTKTDDPQLKREIRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLYSNKSV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 activating transcription factor 1
别名 cAMP-dependent transcription factor ATF-1, Cyclic AMP-dependent transcription factor ATF-1, TREB36
计算分子量 271 aa, 29 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC029619
基因名称 ATF1
Gene ID (NCBI) 466
RRIDAB_3745533
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP18846
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TFs play a vital role in maintaining cell homeostasis by combining DNA regulatory sequences to regulate gene transcription rates. Activating transcription factor 1 (ATF1) is a member of the ATF/CREB family of transcription factors (TFs), specifically interacting with the consensus ATF/CRE site 'TGACGTCA'(PMID: 28032861). ATF1 transcriptionally regulates the expressions of target genes to mediate various cellular processes, and ATF1 effects the progression of tumors (including lung cancer, colorectal cancer,and nasopharyngeal carcinoma).

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