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验证数据展示

  • WB analysis using 86461-2-RR

    WB analysis using 86461-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86461-2-RR (ATAD3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEPG2 using 86461-2-RR

    IF Staining of HEPG2 using 86461-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HEPG2 cells using ATAD3A/B antibody (86461-2-RR, Clone: 251342C9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86461-2-RR

    Affinity and Kinetic Characterization of 86461-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86461-2-RR against Human ATAD3A/B were performed. The affinity constant is 0.654 nM.

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ATAD3A/B Recombinant monoclonal antibody

ATAD3A/B Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86461-2-RR

产品说明书

CloneNo. 251342C9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

ATAD3B, AAA TOB3, AAATOB3, AAA-TOB3, ATAD

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, U-251 cells, mouse kidney tissue, rat kidney tissue, mouse brain tissue
Positive IF/ICC detected inHEPG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86461-2-RR targets ATAD3A/B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9836

Product name: Recombinant human ATAD3B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-270 aa of BC002542

Sequence: MSWLFGVNKGPKGEGAGPPPPLPPAQPGAEGGGDRGLGDRPAPKDKWSNFDPTGLERAAKAARELEHSRYAKEALNLAQMQEQTLQLEQQSKLKEYEAAVEQLKSEQIRAQAEERRKTLSEETRQHQARAQYQDKLARQRYEDQLKQQRLLNEENLRKQEESVQKQEAMRRATVEREMELRHKNEMLRVETEARARAKAERENADIIREQIRLKASEHRQTVLESIRTAGTLFGEGFRAFVTDRDKVTATVAGLTLLAVGVYSAKNATAV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase family, AAA domain containing 3B
别名 ATAD3B, AAA TOB3, AAATOB3, AAA-TOB3, ATAD
计算分子量 72 kDa
观测分子量65-70 kDa
GenBank蛋白编号BC002542
基因名称 ATAD3B
Gene ID (NCBI) 83858
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ5T9A4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATAD3 (ATPase family AAA Domain-containing protein 3) is a mitochondrial membrane bound ATPase whose function has not yet been discovered but its role is essential for embryonic development. The analysis of the cellular properties of ATAD3A and ATAD3B in different human cancer cell lines shows on the contrary that they can present anti-proliferative and chemoresistant properties.

实验方案

Product Specific Protocols
WB protocol for ATAD3A/B antibody 86461-2-RRDownload protocol
IF protocol for ATAD3A/B antibody 86461-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols