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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 86461-2-RR (same clone as 86461-2-PBS)

    WB analysis using 86461-2-RR (same clone as 86461-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86461-2-RR (ATAD3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86461-2-PBS in a different storage buffer formulation.

  • IF Staining of HEPG2 using 86461-2-RR (same clone as 86461-2-PBS)

    IF Staining of HEPG2 using 86461-2-RR (same clone as 86461-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEPG2 cells using ATAD3A/B antibody (86461-2-RR, Clone: 251342C9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86461-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86461-2-RR

    Affinity and Kinetic Characterization of 86461-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86461-2-RR against Human ATAD3A/B were performed. The affinity constant is 0.654 nM.

    {{ptg:ProductFeaturesInfo}}

ATAD3A/B Recombinant monoclonal antibody, PBS Only

ATAD3A/B Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86461-2-PBS

产品说明书

CloneNo. 251342C9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

ATAD3B, AAA TOB3, AAATOB3, AAA-TOB3, ATAD

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86461-2-PBS targets ATAD3A/B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9836

Product name: Recombinant human ATAD3B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-270 aa of BC002542

Sequence: MSWLFGVNKGPKGEGAGPPPPLPPAQPGAEGGGDRGLGDRPAPKDKWSNFDPTGLERAAKAARELEHSRYAKEALNLAQMQEQTLQLEQQSKLKEYEAAVEQLKSEQIRAQAEERRKTLSEETRQHQARAQYQDKLARQRYEDQLKQQRLLNEENLRKQEESVQKQEAMRRATVEREMELRHKNEMLRVETEARARAKAERENADIIREQIRLKASEHRQTVLESIRTAGTLFGEGFRAFVTDRDKVTATVAGLTLLAVGVYSAKNATAV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase family, AAA domain containing 3B
别名 ATAD3B, AAA TOB3, AAATOB3, AAA-TOB3, ATAD
计算分子量 72 kDa
观测分子量65-70 kDa
GenBank蛋白编号BC002542
基因名称 ATAD3B
Gene ID (NCBI) 83858
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ5T9A4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATAD3 (ATPase family AAA Domain-containing protein 3) is a mitochondrial membrane bound ATPase whose function has not yet been discovered but its role is essential for embryonic development. The analysis of the cellular properties of ATAD3A and ATAD3B in different human cancer cell lines shows on the contrary that they can present anti-proliferative and chemoresistant properties.