• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84194-6-RR

    WB analysis using 84194-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84194-6-RR (ATAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84194-6-RR

    IF Staining of HeLa using 84194-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATAD1 antibody (84194-6-RR, Clone: 241449D12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of A431 using 84194-6-RR

    FC experiment of A431 using 84194-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ATAD1 Recombinant antibody (84194-6-RR, Clone:241449D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84194-6-RR

    Affinity and Kinetic Characterization of 84194-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84194-6-RR against Human ATAD1 were performed. The affinity constant is 0.573 nM.

ATAD1 Recombinant antibody

ATAD1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84194-6-RR

产品说明书

CloneNo. 241449D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

FNP001, EC:7.4.2.-, ATPase family AAA domain-containing protein 1, ATAD 1, AFDC1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inLNCaP cells, A549 cells, Jurkat cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84194-6-RR targets ATAD1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ATAD1 fusion protein Ag10343 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase family, AAA domain containing 1
别名 FNP001, EC:7.4.2.-, ATPase family AAA domain-containing protein 1, ATAD 1, AFDC1
计算分子量 361 aa, 41 kDa
观测分子量32-37 kDa
GenBank蛋白编号BC010868
基因名称 ATAD1
Gene ID (NCBI) 84896
RRIDAB_3671746
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8NBU5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mitochondrial AAA (ATPase Associated with diverse cellular Activities) protein ATAD1 (in humans; Msp1 in yeast) removes mislocalized membrane proteins, as well as stuck import substrates from the mitochondrial outer membrane, facilitating their re-insertion into their cognate organelles and maintaining mitochondria's protein import capacity.

实验方案

Product Specific Protocols
WB protocol for ATAD1 antibody 84194-6-RRDownload protocol
IF protocol for ATAD1 antibody 84194-6-RRDownload protocol
FC protocol for ATAD1 antibody 84194-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols