• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84194-6-RR (same clone as 84194-6-PBS)

    WB analysis using 84194-6-RR (same clone as 84194-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84194-6-RR (ATAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84194-6-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84194-6-RR (same clone as 84194-6-PBS)

    IF Staining of HeLa using 84194-6-RR (same clone as 84194-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATAD1 antibody (84194-6-RR, Clone: 241449D12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84194-6-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 84194-6-RR (same clone as 84194-6-PBS)

    FC experiment of A431 using 84194-6-RR (same clone as 84194-6-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ATAD1 Recombinant antibody (84194-6-RR, Clone:241449D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84194-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84194-6-RR

    Affinity and Kinetic Characterization of 84194-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84194-6-RR against Human ATAD1 were performed. The affinity constant is 0.573 nM.

ATAD1 Recombinant antibody, PBS Only

ATAD1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84194-6-PBS

产品说明书

CloneNo. 241449D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

FNP001, EC:7.4.2.-, ATPase family AAA domain-containing protein 1, ATAD 1, AFDC1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84194-6-PBS targets ATAD1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ATAD1 fusion protein Ag10343 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase family, AAA domain containing 1
别名 FNP001, EC:7.4.2.-, ATPase family AAA domain-containing protein 1, ATAD 1, AFDC1
计算分子量 361 aa, 41 kDa
观测分子量32-37 kDa
GenBank蛋白编号BC010868
基因名称 ATAD1
Gene ID (NCBI) 84896
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8NBU5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The mitochondrial AAA (ATPase Associated with diverse cellular Activities) protein ATAD1 (in humans; Msp1 in yeast) removes mislocalized membrane proteins, as well as stuck import substrates from the mitochondrial outer membrane, facilitating their re-insertion into their cognate organelles and maintaining mitochondria's protein import capacity.