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验证数据展示

  • WB analysis of HepG2 using 27602-1-AP (same clone as 27602-1-PBS)

    WB analysis of HepG2 using 27602-1-AP (same clone as 27602-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27602-1-AP (ASPN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27602-1-PBS in a different storage buffer formulation.

  • WB analysis using 27602-1-AP (same clone as 27602-1-PBS)

    WB analysis using 27602-1-AP (same clone as 27602-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27602-1-AP (ASPN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27602-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 27602-1-AP (same clone as 27602-1-PBS)

    IHC staining of human stomach cancer using 27602-1-AP (same clone as 27602-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27602-1-AP (ASPN antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27602-1-PBS in a different storage buffer formulation.

ASPN Polyclonal antibody, PBS Only

ASPN Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27602-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

SLRR1C, PLAP-1, PLAP1, PLAP 1, Periodontal ligament-associated protein 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27602-1-PBS targets ASPN in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag26380

Product name: Recombinant human ASPN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 53-330 aa of BC063114

Sequence: DDEEDNSLFPTREPRSHFFPFDLFPMCPFGCQCYSRVVHCSDLGLTSVPTNIPFDTRMLDLQNNKIKEIKENDFKGLTSLYGLILNNNKLTKIHPKAFLTTKKLRRLYLSHNQLSEIPLNLPKSLAELRIHENKVKKIQKDTFKGMNALHVLEMSANPLDNNGIEPGAFEGVTVFHIRIAEAKLTSVPKGLPPTLLELHLDYNKISTVELEDFKRYKELQRLGLGNNKITDIENGSLANIPRVREIHLENNKLKKIPSGLPELKYLQIIFLHSNSIAR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 asporin
别名 SLRR1C, PLAP-1, PLAP1, PLAP 1, Periodontal ligament-associated protein 1
计算分子量 384 aa, 44 kDa
观测分子量43 kDa
GenBank蛋白编号BC063114
基因名称 ASPN
Gene ID (NCBI) 54829
RRIDAB_3085974
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BXN1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ASPN, also known as periodontal ligament-associated protein1 (PLAP1), is an extracellular matrix (ECM) protein. The calculated molecular weight of ASPN is 43 kDa. It belongs to the small leucine-rich proteoglycans (SLRPs) family and contains a unique aspartate-rich N terminus that distinguishes it from other SLRPs family members. It was firstly identified in human cartilage and it was associated with the pathogenesis of bone and joint diseases, including osteoarthritis, intervertebral disc degeneration and periodontal ligament mineralization (PMID:11152692; 26089687).

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