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验证数据展示

  • WB analysis of K-562 using 68004-1-Ig (same clone as 68004-1-PBS)

    WB analysis of K-562 using 68004-1-Ig (same clone as 68004-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68004-1-PBS in a different storage buffer formulation.

  • IHC staining of rat pancreas using 68004-1-Ig (same clone as 68004-1-PBS)

    IHC staining of rat pancreas using 68004-1-Ig (same clone as 68004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 68004-1-Ig (ASNS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68004-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ASNS antibody included
  • WB analysis using 68004-1-Ig (same clone as 68004-1-PBS)

    WB analysis using 68004-1-Ig (same clone as 68004-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68004-1-PBS in a different storage buffer formulation.

  • IHC staining of rat pancreas using 68004-1-Ig (same clone as 68004-1-PBS)

    IHC staining of rat pancreas using 68004-1-Ig (same clone as 68004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 68004-1-Ig (ASNS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68004-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ASNS antibody included
  • WB analysis of HEK-293 using 68004-1-Ig (same clone as 68004-1-PBS)

    WB analysis of HEK-293 using 68004-1-Ig (same clone as 68004-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68004-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68004-1-Ig (same clone as 68004-1-PBS)

    WB analysis of HeLa using 68004-1-Ig (same clone as 68004-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68004-1-PBS in a different storage buffer formulation.

  • WB analysis of LNCaP using 68004-1-Ig (same clone as 68004-1-PBS)

    WB analysis of LNCaP using 68004-1-Ig (same clone as 68004-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68004-1-PBS in a different storage buffer formulation.

  • WB analysis of A431 using 68004-1-Ig (same clone as 68004-1-PBS)

    WB analysis of A431 using 68004-1-Ig (same clone as 68004-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68004-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 68004-1-Ig (same clone as 68004-1-PBS)

    IF Staining of SH-SY5Y using 68004-1-Ig (same clone as 68004-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using ASNS antibody (68004-1-Ig, Clone: 1C3D3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68004-1-PBS in a different storage buffer formulation.

ASNS Monoclonal antibody, PBS Only

ASNS Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68004-1-PBS

产品说明书

CloneNo. 1C3D3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

1C3D3, Asparagine synthetase [glutamine-hydrolyzing], EC:6.3.5.4, Glutamine-dependent asparagine synthetase, TS11

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ASNS Ready-To-Use IHC Kit. ASNS primary antibody included.

产品信息

68004-1-PBS targets ASNS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6817

Product name: Recombinant human ASNS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 247-561 aa of BC014621

Sequence: LMTDRRIGCLLSGGLDSSLVAATLLKQLKEAQVQYPLQTFAIGMEDSPDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLETYDITTVRASVGMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEEESERLLRELYLFDVLRADRTTAAHGLELRVPFLDHRFSSYYLSLPPEMRIPKNGIEKHLLRETFEDSNLIPKEILWRPKEAFSDGITSVKNSWFKILQEYVEHQVDDAMMANAAQKFPFNTPKTKEGYYYRQVFERHYPGRADWLSHYWMPKWINATDPSARTLTHYKSAVKA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 asparagine synthetase
别名 1C3D3, Asparagine synthetase [glutamine-hydrolyzing], EC:6.3.5.4, Glutamine-dependent asparagine synthetase, TS11
计算分子量 64 kDa
观测分子量 55-64 kDa
GenBank蛋白编号BC014621
基因名称 ASNS
Gene ID (NCBI) 440
RRIDAB_2918751
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08243
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.

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