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验证数据展示

  • WB analysis of K-562 using 68004-1-Ig

    WB analysis of K-562 using 68004-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat pancreas using 68004-1-Ig

    IHC staining of rat pancreas using 68004-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 68004-1-Ig (ASNS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ASNS antibody included
  • WB analysis using 68004-1-Ig

    WB analysis using 68004-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat pancreas using 68004-1-Ig

    IHC staining of rat pancreas using 68004-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 68004-1-Ig (ASNS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ASNS antibody included
  • WB analysis of HEK-293 using 68004-1-Ig

    WB analysis of HEK-293 using 68004-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68004-1-Ig

    WB analysis of HeLa using 68004-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of LNCaP using 68004-1-Ig

    WB analysis of LNCaP using 68004-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 68004-1-Ig

    WB analysis of A431 using 68004-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 68004-1-Ig

    IF Staining of SH-SY5Y using 68004-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using ASNS antibody (68004-1-Ig, Clone: 1C3D3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

ASNS Monoclonal antibody

ASNS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68004-1-Ig

产品说明书

CloneNo. 1C3D3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

1C3D3, Asparagine synthetase [glutamine-hydrolyzing], EC:6.3.5.4, Glutamine-dependent asparagine synthetase, TS11

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, A431 cells, LNCaP cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inrat pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy ASNS Ready-To-Use IHC Kit. ASNS primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68004-1-Ig targets ASNS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6817

Product name: Recombinant human ASNS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 247-561 aa of BC014621

Sequence: LMTDRRIGCLLSGGLDSSLVAATLLKQLKEAQVQYPLQTFAIGMEDSPDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLETYDITTVRASVGMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEEESERLLRELYLFDVLRADRTTAAHGLELRVPFLDHRFSSYYLSLPPEMRIPKNGIEKHLLRETFEDSNLIPKEILWRPKEAFSDGITSVKNSWFKILQEYVEHQVDDAMMANAAQKFPFNTPKTKEGYYYRQVFERHYPGRADWLSHYWMPKWINATDPSARTLTHYKSAVKA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 asparagine synthetase
别名 1C3D3, Asparagine synthetase [glutamine-hydrolyzing], EC:6.3.5.4, Glutamine-dependent asparagine synthetase, TS11
计算分子量 64 kDa
观测分子量 55-64 kDa
GenBank蛋白编号BC014621
基因名称 ASNS
Gene ID (NCBI) 440
RRIDAB_2918751
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP08243
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.

实验方案

Product Specific Protocols
IF protocol for ASNS antibody 68004-1-IgDownload protocol
IHC protocol for ASNS antibody 68004-1-IgDownload protocol
WB protocol for ASNS antibody 68004-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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