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ASNS Monoclonal antibody

ASNS Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C3D3

Cat No : 68004-1-Ig

Print datasheet

Synonyms

ASNS, asparagine synthetase, TS11

验证数据展示

  • WB analysis of K-562 using 68004-1-Ig

    WB analysis of K-562 using 68004-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat pancreas using 68004-1-Ig

    IHC staining of rat pancreas using 68004-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 68004-1-Ig (ASNS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 68004-1-Ig

    WB analysis using 68004-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat pancreas using 68004-1-Ig

    IHC staining of rat pancreas using 68004-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 68004-1-Ig (ASNS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HEK-293 using 68004-1-Ig

    WB analysis of HEK-293 using 68004-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68004-1-Ig

    WB analysis of HeLa using 68004-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of LNCaP using 68004-1-Ig

    WB analysis of LNCaP using 68004-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 68004-1-Ig

    WB analysis of A431 using 68004-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inK-562 cells, A431 cells, LNCaP cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inrat pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68004-1-Ig targets ASNS in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen ASNS fusion protein Ag6817 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name asparagine synthetase
Synonyms ASNS, asparagine synthetase, TS11
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 55-64 kDa
GenBank Accession NumberBC014621
Gene Symbol ASNS
Gene ID (NCBI) 440
RRIDAB_2918751
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08243
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.

实验方案

Product Specific Protocols
WB protocol for ASNS antibody 68004-1-IgDownload protocol
IHC protocol for ASNS antibody 68004-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols