CoraLite® Plus 488-conjugated ASK1 Monoclonal antibody
ASK1 Monoclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1C3A10
验证数据展示
经过测试的应用
| Positive IF detected in | A431 cells |
| Positive FC detected in | A431 cells |
For other applications, we recommend the unconjugated version of this antibody, 67072-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67072 targets ASK1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | FC (Intra), IF/ICC |
| Tested Reactivity | Human, Mouse, Rat |
| Immunogen | ASK1 fusion protein Ag5818 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | mitogen-activated protein kinase kinase kinase 5 |
| Synonyms | ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5 |
| Calculated Molecular Weight | 155 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC054503 |
| Gene Symbol | MAP3K5 |
| Gene ID (NCBI) | 4217 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q99683 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. 67072-1-Ig antibody detects the degraded product of ASK1 in SDS-PAGE.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ASK1 antibody CL488-67072 | Download protocol |
| FC protocol for CL Plus 488 ASK1 antibody CL488-67072 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
